Dopaminergic differentiation of human neural stem cells mediated by co-cultured rat striatal brain slices

Journal of Neurochemistry
Mohammad Raffaqat AnwarMorten Meyer

Abstract

Properly committed neural stem cells constitute a promising source of cells for transplantation in Parkinson's disease, but a protocol for controlled dopaminergic differentiation is not yet available. To establish a setting for identification of secreted neural compounds promoting dopaminergic differentiation, we co-cultured cells from a human neural forebrain-derived stem cell line (hNS1) with rat striatal brain slices. In brief, coronal slices of neonatal rat striatum were cultured on semiporous membrane inserts placed in six-well trays overlying monolayers of hNS1 cells. After 12 days of co-culture, large numbers of tyrosine hydroxylase (TH)-immunoreactive, catecholaminergic cells could be found underneath individual striatal slices. Cell counting revealed that up to 25.3% (average 16.1%) of the total number of cells in these areas were TH-positive, contrasting a few TH-positive cells (<1%) in non-induced areas. The presence of dopamine in the conditioned culture medium was confirmed by HPLC analysis. Interestingly, not all striatal slice cultures induced TH-expression in underlying hNS1 cells. Common to TH-inductive cultures was, however, the presence of degenerating, necrotic areas, suggesting that factors released during ...Continue Reading

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Citations

Nov 19, 2010·Regenerative Medicine·Linda Maria JäderstadEric Herlenius
Aug 7, 2010·Comptes rendus biologies·Karine CharrièreDominique Fellmann
Nov 11, 2016·Nature Communications·Line S ReinertSøren R Paludan
Mar 11, 2017·Archivum Immunologiae Et Therapiae Experimentalis·Michael KoenneckeBarbara Wollenberg

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