Dopaminergic phenotype induced by oestrogens in a human neuroblastoma cell line

The European Journal of Neuroscience
P AgratiA Maggi

Abstract

Oestrogens are the key factor in the sexual differentiation of the mammalian brain and play an important role in the activity of selected areas of the mature brain. To pursue the study of oestrogen action on neural cells at the molecular level, we developed a human neuroblastoma cell line (SK-ER3) expressing the oestrogen receptor (ER). Treatment of these cells with 17beta-oestradiol causes growth arrest and morphological and biochemical differentiation. The aim of the present study was to investigate whether oestrogen-differentiated SK-ER3 neuroblastoma cells acquire the ability to synthesize a specific neurotransmitter and whether the growth arrest previously reported can be ascribed to the blockage of the cells at a specific stage of the cell cycle. The results presented here indicate that oestrogens induce accumulation of SK-ER3 cells in the G0 phase of the cell cycle, underscoring the acquisition of a mature neural phenotype upon hormonal treatment. Most importantly, we show that in the differentiated cells the content of tyrosine hydroxylase and Na+-dependent dopamine uptake is significantly augmented, proving that the oestrogen-differentiated SK-ER3 cells can synthesize and store a specific neurotransmitter. In addition,...Continue Reading

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Citations

Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L WangJ A Gustafsson
Sep 6, 2001·Hormones and Behavior·E KüppersC Beyer
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·C PatroneD Lindholm
Feb 16, 2007·Molecular and Cellular Neurosciences·Sara MerloMaria-Angela Sortino
Mar 15, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A BrusadelliA Maggi
Apr 22, 2009·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Néstor F DíazIván Velasco
Oct 25, 2006·Neurochemical Research·M K Thakur, P K Sharma
Apr 27, 2010·Biochimica Et Biophysica Acta·Magdalena MisiakSusanne Arnold

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