Dopaminergic Receptors and Tyrosine Hydroxylase Expression in Peripheral Blood Mononuclear Cells: A Distinct Pattern in Central Obesity

PloS One
Fernanda LeiteLaura Ribeiro

Abstract

Dopamine (DA) may be involved in central obesity (CO), an inflammatory condition, through its role in the central nervous system and in periphery, where it may affect immune cell function through five different DA receptors (DR). Whether dopaminergic pathways in peripheral immune cells are implicated in the inflammatory condition linked to CO is however unknown. In a cohort of blood donors with and without CO, categorized by waist circumference (WC) (CO: WC ≥ 0.80 m in women and ≥ 0.94 m in men), we studied the expression of DR and tyrosine hydroxylase (TH), the rate-limiting enzyme in the synthesis of DA, in peripheral blood mononuclear cells (PBMCs) and their relation with anthropometric and metabolic/endocrine and inflammatory parameters. DR D1-5 and TH expression was assessed by semi quantitative real-time PCR. As inflammatory markers we investigated the immunophenotype of monocyte subsets by flow cytometry, staining for CD14, CD16, CD11b and CD36. CO individuals showed higher plasma levels of leptin and higher inflammatory pattern of monocytes compared with non-CO. PBMC expression of DR D2, DR D4 and DR D5 as well as of TH were lower in CO in comparison with non-CO. DR D2, and DR D5 expression correlated with lower WC and ...Continue Reading

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Citations

Jun 19, 2018·Psychological Medicine·Fernanda LeiteMarco Cosentino
Jul 13, 2018·Brain and Behavior·Omar Ramos-LopezUNKNOWN MENA Project
Jul 19, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Fernanda Leite, Laura Ribeiro
Jun 5, 2017·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Monica PinoliMarco Cosentino
Apr 24, 2019·European Journal of Nutrition·Andreia GomesLaura Ribeiro
Mar 30, 2021·Frontiers in Psychiatry·Adam WysokińskiKatarzyna Sobierajska

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Methods Mentioned

BETA
ELISA
flow cytometry
PCR
density gradient centrifugation
Assay

Software Mentioned

Predictive Analytics Software
AB Prism 7000 SDS
Paint
CROMSYSTEMS
- Gate Pro
PASW Statistics
Cell Quest

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