Dorsal and ventral positional cues required for the onset of planarian regeneration may reside in differentiated cells

Developmental Biology
K KatoKiyokazu Agata

Abstract

We previously showed by grafting experiments that the dorsoventral (DV) interaction evokes morphogenetic events similar to those that occur in regeneration. However, it is not yet understood whether the stem cells themselves or differentiated cells have the ability to induce regeneration. Here we demonstrated by a combination of X-ray irradiation and grafting experiments that the dorsal and ventral positional cues inducing morphogenetic events are retained in X-ray-irradiated tissues, suggesting that the differentiated cells may be responsible for the positional cues. We grafted a small piece of irradiated worm, in which the stem cells were certainly eliminated, to an intact one in DV-reversed orientation. We observed that projections were developed from the host-donor boundary, as in the previous experiments. Whole-mount in situ hybridization with several markers demonstrated that the projections had a newly established DV axis and also had anterior or posterior characteristics. Furthermore, chimeric analysis with a strain-specific marker showed that the projections consisted of nonirradiated cells and that IFb-expressing cells, which normally belonged to the ventral tissue, could be generated even from the stem cells located ...Continue Reading

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Citations

Sep 26, 2002·Developmental Biology·Kazuya OgawaKiyokazu Agata
Dec 16, 2004·Development Genes and Evolution·Albert CardonaRafael Romero
Nov 25, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M KalafatićV Besendorfer
May 21, 2004·Nature Reviews. Neuroscience·Hans Meinhardt
Jun 13, 2002·Development, Growth & Differentiation·Kazuya OgawaKiyokazu Agata
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Shigenobu YazawaKiyokazu Agata
Apr 1, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Kiyokazu Agata, Yoshihiko Umesono
May 16, 2007·Development, Growth & Differentiation·Hidefumi Orii, Kenji Watanabe
Jan 19, 2010·Development, Growth & Differentiation·Norito ShibataKiyokazu Agata
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Peter W Reddien, Alejandro Sánchez Alvarado
Jul 5, 2008·Annual Review of Cell and Developmental Biology·Jeremy P Brockes, Anoop Kumar
Oct 11, 2003·Current Opinion in Genetics & Development·Kiyokazu Agata
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Sep 25, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tomoko F ShibataKiyokazu Agata
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Aug 21, 2013·Cell Reports·Jessica N WitchleyPeter W Reddien
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Jun 30, 2006·Seminars in Cell & Developmental Biology·Kiyokazu AgataYoshihiko Umesono
Jan 1, 2013·Plants·Miyuki Nakata, Kiyotaka Okada
Jan 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yumi SaitoKiyokazu Agata
Jan 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K AgataY Saitoh
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