Dorsal root ganglion neurons respond with prolonged extracellular signal-regulated protein kinase phosphorylation following noxious heat and cold stimulation

Neuroscience Letters
Josef Donnerer, Ingrid Liebmann

Abstract

In the present study the time course of extracellular signal-regulated protein kinase phosphorylation (pERK1/2 appearance) in lumbar sensory dorsal root ganglia (DRG) was determined following a 5-min noxious heat or a noxious cold stimulus to the hind paw of the rat. The thermal stimuli were chosen to activate transient receptor potential (TRP) channels, but not to induce tissue damage. A quantitative analysis of phospho-ERK1/2 was performed by protein extraction and Western blot analysis. Western blot analysis showed that following the heat stimulus, phosphorylation of ERK1/2 increased 2-3-fold between 10 and 30min in the DRG on the ipsilateral side. High levels were maintained from 30min up to 16h. Following the cold stimulus to the paw, pERK1/2 immediately increased 2-fold within 2min in the DRG on the ipsilateral side, it declined within 2h and reached a second peak at 4h. In the DRGs on the contralateral side of the paw's heat or cold immersion the pERK1/2 remained low at all time points investigated. Fluorescence immunohistochemistry of the DRG following the thermal stimuli revealed an increased cytoplasmic staining for pERK1/2 in neurons. The present results show that following a 5-min nociceptive thermal stimulus sensor...Continue Reading

References

Mar 26, 2004·Life Sciences·Koichi Obata, Koichi Noguchi
Mar 30, 2004·Neuron·Michael BandellArdem Patapoutian
Sep 24, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zhi-Ye ZhuangRu-Rong Ji
Aug 20, 2005·The Journal of Clinical Investigation·Koichi ObataKoichi Noguchi
Jan 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yuji KarashimaThomas Voets
Jan 20, 2009·Brain Research Reviews·Ru-Rong JiGary R Strichartz

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