Dose- and Time-Dependent In Vitro Effects of Divalent and Trivalent Iron on the Activity of Bovine Spermatozoa

Biological Trace Element Research
Eva TvrdáPeter Massanyi

Abstract

This in vitro study was designed to assess the impact of divalent (Fe(2+)) or trivalent (Fe(3+)) iron on the activity and oxidative balance of bovine spermatozoa at specific time intervals (0, 2, 8, 16, and 24 h) during an in vitro culture. Forty-five semen samples were collected from adult breeding bulls and diluted in physiological saline solution supplemented with different concentrations (0, 1, 5, 10, 50, 100, 200, 500, 1000 μmol/L) of FeCl2 or FeCl3. Spermatozoa motion parameters were assessed using the SpermVision™ computer-aided sperm analysis (CASA) system. Cell viability was examined with the metabolic activity 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the nitroblue-tetrazolium (NBT) test was applied to quantify the intracellular superoxide formation. Both divalent and trivalent iron exhibited a dose- and time-dependent impact on the spermatozoa physiology and oxidative balance. Concentrations ≥50 μmol/L FeCl2 and ≥100 μmol/L FeCl3 led to a significant decrease of spermatozoa motility (P < 0.05) and mitochondrial activity (P < 0.001 with respect to 200-1000 μmol/L FeCl2/FeCl3; P < 0.01 in case of 100 μmol/L FeCl2/FeCl3), accompanied by a significant superoxide overproduction (P < 0.0...Continue Reading

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Citations

Dec 17, 2009·Acta Veterinaria Scandinavica·Monika MartiniakováPeter Chrenek
Sep 8, 2016·Journal of Animal Science and Biotechnology·Eva TvrdáNorbert Lukáč
Dec 18, 2019·Environmental Science and Pollution Research International·Pushpa Rani GuvvalaSellappan Selvaraju
Feb 12, 2017·Acta Veterinaria Scandinavica·Maja Zakošek PipanPetra Zrimšek
Jun 3, 2021·International Journal of Molecular Sciences·Eva TvrdáEliška Gálová

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