Dose analysis of photobiomodulation therapy on osteoblast, osteoclast, and osteocyte

Journal of Biomedical Optics
Sungsoo NaJie Chen

Abstract

The objective of this study was to evaluate the effects of varying light doses on the viability and cellular activity of osteoblasts, osteocytes, and osteoclasts. A light application device was developed to apply 940-nm wavelength light from light-emitting diodes on three cultured cells, MC3T3-E1, MLO-A5, and RANKL-treated RAW264.7 cells. The doses (energy density) on cells were 0, 1, 5, and 7.5  J  /  cm2. The corresponding light power densities at the cell site were 0, 1.67, 8.33, and 12.5  mW  /  cm2, respectively, and the duration was 10 min. The results showed that the three cell types respond differently to light and their responses were dose dependent. Low-dose treatment (1  J  /  cm2) enhanced osteoblast proliferation, osteoclast differentiation, and osteoclastic bone resorption activity. Osteocyte proliferation was not affected by both low- and high-dose (5  J  /  cm2) treatments. While 1  J  /  cm2 did not affect viability of all three cell types, 5  J  /  cm2 significantly decreased viability of osteocytes and osteoclasts. Osteoblast viability was negatively impacted by the higher dose (7.5  J  /  cm2). The findings suggest that optimal doses exist for osteoblast and osteoclast, which can stimulate cell activities, a...Continue Reading

References

Mar 29, 2006·Lasers in Medical Science·Norihito AiharaKazutaka Kasai
Mar 11, 2009·Journal of Biomedical Science·Xuejuan Gao, Da Xing
Mar 12, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Pierangelo AngelettiLydia Masako Ferreira
Jan 25, 2011·Photomedicine and Laser Surgery·Marinês Vieira da Silva SousaFernanda Angelieri
Feb 22, 2012·Lasers in Medical Science·Priscila D'Andrea FonsecaSolange de Paula Ramos
Mar 3, 2012·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Gauri Doshi-Mehta, Wasundhara A Bhad-Patil
Apr 3, 2012·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Fernanda CeperaDanilo F Siqueira
Mar 22, 2013·Lasers in Medical Science·Carla Roberta TimAna Claudia Muniz Rennó
Aug 7, 2013·Lasers in Medical Science·Mohsen ShiraziNasim Chiniforush
Mar 7, 2014·Lasers in Medical Science·Mario MigliarioFilippo Renò
Apr 8, 2014·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Kwang Heung HanYoon-Ah Kook
May 29, 2014·Acta ortopedica brasileira·Danillo BarbosaRenato Aparecido de Souza
Jun 7, 2014·Photomedicine and Laser Surgery·Farzad FazilatAntonio Luiz Barbosa Pinheiro
Oct 8, 2014·European Journal of Orthodontics·Tanya J FranzenVaska Vandevska-Radunovic
Mar 16, 2017·Lasers in Medical Science·Sevin Erol ÜretürkErhan Fıratlı
Apr 10, 2017·Archives of Oral Biology·M MilliganS-G Gong

❮ Previous
Next ❯

Citations

May 20, 2020·Photobiomodulation, Photomedicine, and Laser Surgery·Weyland ChengWeili Li
Oct 6, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Chuan-Tsung SuJih-Huah Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.

Related Papers

Current Osteoporosis Reports
Evangelia KalaitzoglouKathryn M Thrailkill
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology
Li-li ChenYan-min Wu
© 2022 Meta ULC. All rights reserved