Dose and chemical species-specific effects of selenium against arsenite toxicity in cultured hepatocytes of rainbow trout (Oncorhynchus mykiss)

Metallomics : Integrated Biometal Science
Ankur Jamwal, Som Niyogi

Abstract

The present study evaluated the mechanistic underpinnings of the interactive effects of selenium (Se), both inorganic (selenite) and organic (selenomethionine (SeMet)), against arsenite (As-III) cytotoxicity using rainbow trout (Oncorhynchus mykiss) hepatocytes in primary culture. Arsenite is known to induce cytotoxic effects by disrupting cellular redox homeostasis. In contrast, Se is essential for the maintenance of cellular anti-oxidative machinery, but when present above a threshold concentration, can also induce reactive oxygen species (ROS) generation and cause oxidative damage. In this study, hepatocytes were exposed to 100 μM arsenite independently or in combination with selenite or SeMet (5-40 μM) for 24 h. Exposure to arsenite alone reduced cell viability by inducing intracellular ROS generation, which also corresponded with a concomitant decrease in cellular thiol (GSH : GSSG) ratio and the activities of enzymatic antioxidants (GPx and SOD). Both selenite and SeMet were found to ameliorate the arsenite-induced loss of cell viability and thiol balance significantly, but only at low-intermediate exposure levels (5-20 μM), with selenite being more effective than SeMet. Further analyses of cellular antioxidative pathways...Continue Reading

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