PMID: 15252286Jul 15, 2004Paper

Dose-dependent activation of Ca2+-activated K+ channels by ethanol contributes to improved endothelial cell functions

Alcoholism, Clinical and Experimental Research
Christoph Rüdiger Wolfram KuhlmannJ Wiecha

Abstract

Regular moderate alcohol (EtOH) intake seems to protect against both coronary artery disease and ischemic stroke, whereas the risk increases with heavy EtOH consumption. Effects of EtOH on endothelial cell function may be relevant to these disparate effects. Potassium channels play an important role in the regulation of endothelial cell functions. Therefore, we investigated whether Ca-activated K channels (BKCa) are modulated by EtOH. Furthermore, we examined whether EtOH-induced changes of endothelial nitric oxide (NO) formation and cell proliferation are due to BKCa activation. The patch-clamp technique was used to investigate BKCa activity in cultured human umbilical vein endothelial cells (HUVEC). NO formation was analyzed by using the fluorescence dye 4,5-diaminofluorescein. Endothelial proliferation was examined by using cell counts and measuring [H]thymidine incorporation. EtOH dose-dependently (10-150 mmol/liter) modulated BKCa-activity, with the highest increase of open-state probability at a concentration of 50 mmol/liter (n = 13; p < 0.05). Inside-out recordings revealed that this effect was due to direct BKCa activation, whereas open-state probability was not changed in cell-attached recordings after pertussis toxin...Continue Reading

References

Jan 1, 1992·The Journal of Physiology·B Nilius, W Wohlrab
Oct 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D J AdamsC Van Breemen
Jul 1, 1989·Circulation·P M Vanhoutte, H Shimokawa
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Nov 1, 1995·Stroke; a Journal of Cerebral Circulation·W G Mayhan, S P Didion
Aug 1, 1994·Alcoholism, Clinical and Experimental Research·K HatakeS Hishida
Mar 12, 1999·Journal of Molecular and Cellular Cardiology·H Drexler, B Hornig
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·C H ChenD Mochly-Rosen
Nov 24, 1999·The New England Journal of Medicine·K BergerC H Hennekens
Sep 26, 2000·American Journal of Physiology. Cell Physiology·F S WaltersJ S Ellingson
Nov 9, 2000·Addiction·G CorraoK Poikolainen
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·H Sun, W G Mayhan
Oct 3, 2001·Physiological Reviews·B Nilius, G Droogmans
Oct 20, 2001·The Journal of Biological Chemistry·Noriyuki SuzukiTetsuo Nagano
Feb 7, 2003·American Journal of Physiology. Cell Physiology·Alejandro M Dopico

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Citations

May 23, 2008·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Xiao-Chen RuQiang Xia
Jun 5, 2010·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Noboru Toda, Kazuhide Ayajiki
Mar 1, 2007·Vascular Health and Risk Management·Sudesh VasdevPawan K Singal
May 19, 2012·Nutrients·Paul A Cahill, Eileen M Redmond
Dec 12, 2007·Alcoholism, Clinical and Experimental Research·J Koudy WilliamsShay Soker
Jan 18, 2005·Vascular Pharmacology·Christoph Rüdiger Wolfram KuhlmannJohannes Wiecha
Dec 14, 2011·Vascular Pharmacology·Juliana T RochaCarlos R Tirapelli
Dec 7, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ramón RodrigoLeonardo Vergara
Apr 16, 2008·Alcohol·Joanna GoralElizabeth J Kovacs
Sep 14, 2007·Alcoholism, Clinical and Experimental Research·Mark S BrodieAlejandro M Dopico
May 11, 2006·Molecular and Cellular Biochemistry·Sudesh VasdevVeeresh Gadag
Mar 31, 2016·Journal of Atherosclerosis and Thrombosis·Aoi TanakaUNKNOWN CIRCS Investigators
Apr 21, 2017·Turkish Journal of Medical Sciences·Havva KubatKansu Büyükafşar
Oct 17, 2020·Oxidative Medicine and Cellular Longevity·Andy W C ManNing Xia

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