Dose-dependent cellular and humoral responses in Galleria mellonella larvae following beta-glucan inoculation

Microbes and Infection
Peter MowldsKevin Kavanagh

Abstract

Galleria mellonella larvae were inoculated with different doses of beta-glucan by injection into the haemocoel. Those larvae that had received high doses of beta-glucan (15, 30 or 60microg/larva) demonstrated increased survival following infection with the yeast Candida albicans. High concentrations of glucan induced an increase in haemocyte density and a reduction in yeast proliferation within the haemocoel. Proteomic analysis of glucan-treated larvae revealed increased expression of a variety of peptides some of which may possess antimicrobial properties. Analysis of expression profiles revealed that low doses of beta-glucan (3.75microg/larva) triggered the increased expression of certain peptides (e.g. hemolin) while high dose inoculation was required before the increased expression of others (e.g. archaemetzincin) was evident. These results indicate that low doses of beta-glucan induce a limited immune response while high doses induce an immune response that has the potential to curtail the threat within the haemocoel but also withstand a subsequent infection. Immune priming gives insects the ability to withstand a potentially lethal infection if exposed to a low level of the pathogen 24-48h previously. Immune priming has r...Continue Reading

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Citations

Aug 10, 2012·Biology Letters·Seanna J McTaggartTom J Little
Feb 25, 2014·Journal of Insect Physiology·Niall BrowneKevin Kavanagh
Jul 20, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Niall BrowneKevin Kavanagh
Jun 19, 2010·Developmental and Comparative Immunology·Pawel MakMałgorzata Cytryńska
Mar 13, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Miguel Moreno-GarcíaHumberto Lanz-Mendoza
Jul 26, 2016·Frontiers in Microbiology·Valeria VargasHumberto Lanz-Mendoza
Apr 6, 2017·Pigment Cell & Melanoma Research·Miranda M A Whitten, Christopher J Coates
Apr 13, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jenson LimRobin C May
Feb 22, 2018·ChemMedChem·Marine BlanchetJean Michel Brunel
Feb 23, 2020·Virulence·Gerard SheehanKevin Kavanagh
Oct 26, 2018·Frontiers in Microbiology·Gheniffer FornariSybren de Hoog
Feb 6, 2016·Insect Science·Iwona Wojda
Nov 15, 2018·Cell Biology and Toxicology·Christopher J CoatesWill Layton
Nov 30, 2018·Journal of Fungi·Thais Cristine PereiraLiliana Scorzoni
Jul 5, 2019·Archives of Toxicology·Helena EmeryChristopher J Coates
Dec 28, 2018·Journal of Fungi·Nuria Trevijano-Contador, Oscar Zaragoza
Aug 28, 2020·Frontiers in Cellular and Infection Microbiology·Rodnei Dennis RossoniEleftherios Mylonakis
Sep 25, 2020·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Monalessa Fábia Pereira, Ciro César Rossi
Nov 25, 2020·Pathogens and Disease·Sylwia StączekMałgorzata Cytryńska
Mar 5, 2021·Journal of Insect Physiology·Gerard SheehanKevin Kavanagh
Jun 20, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Helena EmeryChristopher J Coates

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