Dose-dependent effects of ouabain on spiral ganglion neurons and Schwann cells in mouse cochlea

Acta Oto-laryngologica
Zhi-Jian ZhangQing-Quan Hua

Abstract

This study aimed in fully investigating the toxicities of ouabain to mouse cochlea and the related cellular environment, and providing an optimal animal model system for cell transplantation in the treatment of auditory neuropathy (AN) and sensorineural hearing loss (SNHL). Different dosages of ouabain were applied to mouse round window. The auditory brainstem responses and distortion product otoacoustic emissions were used to evaluate the cochlear function. The immunohistochemical staining and cochlea surface preparation were performed to detect the spiral ganglion neurons (SGNs), Schwann cells and hair cells. Ouabain at the dosages of 0.5 mM, 1 mM and 3 mM selectively and permanently destroyed SGNs and their functions, while leaving the hair cells relatively intact. Ouabain at 3 mM resulted in the most severe SGNs loss and induced significant loss of Schwann cells started as early as 7 days and with further damages at 14 and 30 days after ouabain exposure. The application of ouabain to mouse round window induces damages of SGNs and Schwann cells in a dose- and time-dependent manner, this study established a reliable and accurate animal model system of AN and SNHL.

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Citations

Jan 27, 2019·Experimental and Therapeutic Medicine·Ming GuanXia Gao
Aug 29, 2018·BioMed Research International·Timo SchomannMargriet A Huisman
Dec 29, 2020·Neuroscience Letters·Xi WangYang Yang
Sep 10, 2021·Cell Transplantation·Tetsuji Sekiya, Matthew C Holley

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