PMID: 7542030Jun 20, 1995Paper

Dose-dependent elevation of cyclic AMP, activation of glycogen phosphorylase, and release of lactate by amylin in rat skeletal muscle

Biochimica Et Biophysica Acta
R PittnerT Rink

Abstract

We report here our investigation of the role of cyclic AMP (cAMP) in amylin signal transduction in isolated strips of soleus muscle. Rat amylin, at 100 nM, increased cAMP levels, from 0.431 +/- 0.047 to a peak of 1.24 +/- 0.01 pmol cAMP/mg wet wt. after 5 min, in the absence of added phosphodiesterase inhibitor. The EC50 of the response was 0.48 nM (+/- 0.12 log units) in the absence of insulin and 0.3 nM (+/- 0.18 log units) in the presence of 7.1 nM insulin. The response seen with a maximally effective concentration of amylin (10 nM) was similar to that seen with a maximally effective concentration of epinephrine (1 microM) under the same conditions. Consistent with the observed rise in cAMP there was an increase in glycogen phosphorylase a (EC50 2.2 nM +/- 0.25 log units), decreased glycogen content (EC50 0.9 nM +/- 0.22 log units) and enhanced production of lactate (EC50 1.5 nM +/- 0.33 log units). These data support the concept that amylin promotes glycogenolysis in skeletal muscle and enhances production of lactate through glycolysis as a result of activation of Gs coupled receptors, stimulation of adenylate cyclase, elevation of cAMP levels and activation of glycogen phosphorylase.

References

Mar 1, 1978·Analytical Biochemistry·D LeBelA R Beaudoin
Sep 1, 1978·The American Journal of Physiology·K M BaldwinR E Lewis
May 9, 1992·Lancet·J E KodaL G Linarelli
Jun 14, 1991·Biochemical and Biophysical Research Communications·G C ZhuA R Saltiel
Aug 1, 1991·European Journal of Biochemistry·S NakielnyP Cohen
Jan 31, 1991·Biochemical and Biophysical Research Communications·R O DeemsD A Young
Mar 1, 1990·The Journal of Clinical Investigation·A OgawaK L Luskey
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G J CooperK B Reid
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G J CooperK B Reid
Feb 16, 1987·European Journal of Biochemistry·R A ChallissE A Newsholme
May 1, 1985·The American Journal of Physiology·D A YoungJ O Holloszy
Jan 1, 1994·Journal of Cellular Biochemistry·R A PittnerT J Rink

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Citations

Sep 16, 1999·FEBS Letters·Y WangG J Cooper
May 25, 1999·Metabolism: Clinical and Experimental·M C MooreA D Cherrington
Nov 14, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·T RiedigerE Simon
Sep 2, 1998·The American Journal of Physiology·A L CastleJ L Ivy
Feb 12, 1998·The American Journal of Physiology·A L CastleJ L Ivy

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