Dose-dependent immunomodulatory effects of acetylsalicylic acid and indomethacin in human whole blood: potential role of cyclooxygenase-2 inhibition

Scandinavian Journal of Immunology
C HärtelC Schultz

Abstract

The aim of the study was to characterize the in vitro effect of non-steroidal anti-inflammatory drugs (NSAIDs) on the production of pro-inflammatory cytokines in a human whole blood assay. Whole blood samples were pre-incubated with acetylsalicylic acid, indomethacin, selective cyclooxygenase (COX)-1 inhibitor (SC-560), COX-2 inhibitor (NS-398) or prostaglandin E2 (PGE2) before stimulation with lipopolysaccharide (LPS). Pro-inflammatory and anti-inflammatory cytokines were determined directly at the cell level with the help of flow cytometry and/or in the plasma supernatant with the help of ELISA. High doses of acetylsalicylic acid were needed to inhibit pro-inflammatory cytokine production. In contrast, low-to-moderate doses induced a modestly enhanced production of pro-inflammatory cytokines. Moreover, indomethacin was demonstrated to increase the expression of interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha) in a dose-dependent fashion. Upon addition of PGE2, however, LPS-induced IL-6 and TNF-alpha production was suppressed regardless of indomethacin presence. Interestingly, selective COX-2 inhibition (NS-398), but not selective COX-1 inhibition (SC-560), exerted a stimulatory effect on the expression of pro...Continue Reading

References

Feb 1, 1985·Arthritis and Rheumatism·M J Palmoski, K D Brandt
Aug 12, 1994·Science·E Kopp, S Ghosh
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·J L MasferrerK Seibert
Nov 1, 1996·The Journal of Clinical Investigation·B K ReuterJ L Wallace
Nov 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M R Forwood
Nov 5, 1997·The Journal of Biological Chemistry·J A Williams, E Shacter
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·C J SmithP C Isakson
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·X M XuK K Wu
Jun 17, 1999·Nature Medicine·D W GilroyD A Willoughby
Jan 22, 2000·Arthritis and Rheumatism·L J CroffordL B van de Putte
Mar 15, 2000·Circulation·E H Awtry, J Loscalzo
Nov 23, 2000·The New England Journal of Medicine·C BombardierUNKNOWN VIGOR Study Group
Mar 22, 2001·The New England Journal of Medicine·E H Choy, G S Panayi

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Citations

Mar 30, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Manuel Lima-RodriguesArmando Almeida
May 11, 2005·Neuroscience Letters·C MarchiniE Fioretti
Apr 28, 2009·Cellular Immunology·Bruno L OliveiraJosé Alexandre M Barbuto
Aug 28, 2007·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·E YazarM Elmas
Jul 27, 2010·International Journal of Cancer. Journal International Du Cancer·Annelinde TerlouLeen J Blok
Oct 14, 2016·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Kashmira WaniNitin Shasikant Chouthai
May 30, 2015·Journal of Lipid Research·Jiao SongMarcos E Milla
Feb 22, 2008·Clinical Pharmacology and Therapeutics·X-M WangR A Dionne
Jan 29, 2020·The Clinical Journal of Pain·Yuan Z LimSultana Monira Hussain

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