PMID: 11326640May 1, 2001Paper

Dose-response relationships on the expression profile of cytokeratins and vimentin in rat submandibular glands following fractionated irradiation

Anticancer Research
S Bartel-FriedrichR Moll

Abstract

The extent of radiogenic damage in salivary gland (SG) tissue depends on the radiation dose (RD), the fractionation (FN) and the localization of SG in the radiation field (RF). While the functional restriction and the radiogenic SG tissue damage are well documented using histomorphological, electron-microscopic and enzyme-histochemical methods, immunohistochemical analysis (IH) of cytokeratins (CK), epithelial differentiation markers, and vimentin, a marker of mesenchymal cells, are rare. Previous studies have shown stronger immunoreactivities of CK in irradiated glands exposed to 60 Gy total dosage. This study was performed to examine dose dependence and alterations related to age, RF, and latency of irradiation. In 124 rat mandibular SG we investigated the vimentin and CK staining profile dependent on age [1 year (y) vs. 1 1/2 y], on FN [2 Gy/day up to a total dosage of 20/40/60 Gy (x-rays)], on RF (inside vs. outside RF) and on the time since irradiation (1/2 y vs. 1 y) using IH. The mouse monoclonal anti-CK antibodies [(AB) D5/16B4, Ks 13.1, E 3, K8.12, Ks 18.04, against CK 5-6, CK 13, CK 17, CK 13-15-16, CK 18) and the polyclonal anti-vimentin AB GP53 identified different epithelia and mesenchymal structures in rat SG tiss...Continue Reading

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