Dose-response studies involving controlled deposition of less than 100 particles generated and levitated in an ac trap onto lung cells, in vitro, and quantitation of ICAM-1 differential expression

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Allen E HaddrellGeorge R Agnes

Abstract

A developing area of interest regarding the relationship between the adverse health effects associated with particles suspended in the troposphere is an understanding of how particle chemical composition influences different biological outcomes. Described is the development and application of an apparatus and methodology wherein a known number of particles of tropospherically relevant chemical composition can be designed and levitated in an alternating current (ac) trap followed by their controlled deposition directly from the ac trap onto air-liquid interface cultured lung cells. A downstream biological response, differential upregulation of intercellular adhesion molecule (ICAM)-1, was measurable using fluorescence microscopy in the air-liquid interface human lung cell cultures even though the dose per culture was 0-100 lipopolysaccharide (LPS)-containing elemental carbon particles (52 pg LPS per 6.3 microm diameter particle). Fluorescence emission intensity data measured from a 1 mm2 area centered over the site of particle deposition were fitted using a least squares linear regression line. Because the total mass of each different compound comprising each of the particles delivered to the culture was known, the data generate...Continue Reading

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Citations

Sep 29, 2011·Toxicology and Applied Pharmacology·Ryohei Miyata, Stephan F van Eeden
Apr 9, 2009·Respirology : Official Journal of the Asian Pacific Society of Respirology·James C Hogg, Stephen van Eeden
Nov 4, 2008·American Journal of Respiratory Cell and Molecular Biology·Rachel L ZemansGregory P Downey

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