PMID: 2490400Jan 1, 1989Paper

Dot-enzyme immunoassay for visual detection of Brucella melitensis antibodies

Giornale Di Batteriologia, Virologia Ed Immunologia
M S LeonardiD Gazzara

Abstract

An indirect enzyme-linked immunosorbent assay, using antigen coupled to paper, has been adapted for the detection of Brucella melitensis antibodies. Optimum conditions were achieved by incubation of 1 ml of diluted serum with a single piece of paper coated with purified Brucella antigens for a period of one hour, and by addition of a goat anti-human enzyme conjugate antibody for one hour again. Under these conditions 80 human sera were examined and the results obtained were compared with Wright agglutination test.

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