Dot immunobinding assay for detection of bovine herpesvirus 4 antibodies in rabbits

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
M EssmailA A Hegazy

Abstract

A dot immunobinding assay (DIA) was used for the detection of antibody against bovine herpesvirus 4 (BHV-4) in experimentally infected specific-pathogen-free male and female rabbits. A semipurified virus preparation was used as the antigen, and protein A/G-horseradish-peroxidase conjugate and diaminobenzidine tetrahydrochloride were used as the detection system. Results of the DIA procedure on serum samples of experimentally infected male and female rabbits were compared with those of a complement-dependent virus neutralization (VN) test. None of the tested sera (0/60 samples) from either male or female rabbits were positive by the complement-dependent VN test. Results of the DIA procedure for the same tested sera were positive in 35 of 60 samples (58%) from BHV-4 infected rabbits, indicating higher sensitivity of DIA procedure as compared with the complement-dependent VN test.

References

Apr 1, 1990·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·K Naeem, S M Goyal
Sep 1, 1987·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·G CastrucciR Gatti
Jan 1, 1986·Comparative Immunology, Microbiology and Infectious Diseases·G CastrucciE Bordoni
Jan 1, 1974·Archiv für die gesamte Virusforschung·L N Potgieter, C J Maré
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon

Citations

May 13, 2015·Revista Argentina de microbiología·Pedro MoranAndrea E Verna
Jan 16, 2020·Journal of Veterinary Science·Florencia RomeoAndrea E Verna

Related Concepts

Metazoa
Antibodies, Viral
Bos indicus
Cattle Diseases
Neutralization Tests
Chinchilla Rabbits
Statistical Sensitivity
Serologic Tests
Immunoblotting, Reverse
Herpesvirus 16, Cercopithecine

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