Dot immunobinding assay for detection of mite antigens in house-dust samples

Journal of Medical Entomology
G MistrelloP Falagiani

Abstract

A new test was developed specifically to detect mite antigens in house-dust. It uses a nitrocellulose dipstick spotted with specific antimite antibodies that act as a capture matrix; the same antibodies act as a detecting reagent when conjugated with colloidal dye particles. Aclotest is a 1-step assay, where a spotted dipstick is placed in a tube containing the detecting reagent and the house-dust sample. No instrumentation or previous extraction procedure of the sample is required, and the test response is visible as a colored spot, after 1 h incubation. The sensitivity and specificity of the new test were compared with those of Acarex and Der p1/Der f1 ELISA tests.

Citations

Feb 7, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A TsayUNKNOWN Multi-Centre Study Group
May 10, 2011·Revue des maladies respiratoires·J-C Bessot, G Pauli
Feb 9, 1999·The Journal of Allergy and Clinical Immunology·E Tovey, G Marks
Nov 20, 2016·Current Allergy and Asthma Reports·Anne CassetFrédéric de Blay

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