Double determination of long noncoding RNAs from lung cancer via multi-amplified electrochemical genosensor at sub-femtomole level

Biosensors & Bioelectronics
Xiaoyan LiHe Huang

Abstract

An ultrasensitive electrochemical genosensor has been fabricated for the double determination of two different specific sequences deduced from the maternally expressed gene3 (MEG3) lncRNA (long noncoding RNA), which was demonstrated by coupling RNase A-aided target recycling with DNA supersandwich-induced signal enhancement, based on a composite interface of graphene-like tungsten disulfide/dendritic gold nanostructures (WS2/DGN). Firstly, duple target sequences of T1 and T2 were captured by the primer probes of P1/P2 functionalized Fe3O4@C magnetic nanoparticles, via the DNA/RNA hybridization between T1/T2 and P1/P2. In the presence of RNase A, T1 and T2 were released to trigger the target recycling, accompanied by the generation of numerous intermediate DNAs designated as IT1 and IT2, respectively. After the magnetic separation, the IT1 and IT2 were liberated and hybridized with the capture probes of CP1/CP2 loaded DGN/WS2 modified electrode. Subsequently, the stepwise DNA hybridization chain reactions (HCR) labeled with ferrocene (Fc) and methyleneblue (MB) were processed, respectively. The DPV current values of Fc and MB were recorded, which were proportional with the concentration of T1 and T2, respectively. Using the mult...Continue Reading

Citations

Jan 27, 2019·Analytical and Bioanalytical Chemistry·Rebeca Miranda-CastroMaría Jesús Lobo-Castañón
Apr 23, 2020·Journal of Nanobiotechnology·Congli TangNongyue He
Sep 7, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Miyuki Tabata, Yuji Miyahara
Dec 4, 2020·Emerging Topics in Life Sciences·Ainoa GuinartTeresa D Tetley
Apr 8, 2020·Analytica Chimica Acta·S CampuzanoJ M Pingarrón

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