PMID: 11906608Mar 22, 2002Paper

Double dimer peptide constructs are immunogenic and protective against Plasmodium falciparum in the experimental Aotus monkey model

The Journal of Peptide Research : Official Journal of the American Peptide Society
Z RiveraM E Patarroyo

Abstract

Multiple antigen peptide constructs (MAPs) have been used to obtain defined multimeric peptide molecules useful in the development of possible synthetic malaria vaccines. In this context, a method was developed, named double dimer constructs (DDCs), involving the direct synthesis of a dimeric peptide with a C-terminal cysteine. A tetrameric molecule was then obtained by oxidation of sulfhydryl groups. Dimer synthesis was optimized using a Fmoc/tBu strategy, dimers were purified by HPLC, oxidized with DMSO and characterized by HPLC and MALDI-TOF-MS. The tetramers or DDCs obtained by this method were used as immunogens in the search for a possible malaria vaccine. It was found that they were immunogenic in the experimental Aotus monkey model, and were able to induce protective immunity when challenged experimentally with a highly infective Plasmodium falciparum malaria strain.

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Citations

Sep 15, 2004·Immunogenetics·Fabio CastilloCarlos Parra-López
Jul 31, 2016·Journal of Molecular Graphics & Modelling·Sonia M Aguilera-SeguraAndrés Fernando González Barrios
Oct 1, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·Petr NiederhafnerJan Jezek
Sep 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nubia F BarreraJulio C Calvo
Nov 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yahson F VarelaManuel Elkin Patarroyo

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