PMID: 1194358Nov 1, 1975Paper

Double labeling with [3H]thymidine and [125I]iododeoxyuridine as a method for determining the fate of injected DNA and cells in vivo

The Journal of Cell Biology
D K Myers, L E Feinendegen

Abstract

Mice were injected intravenously and intraperitoneally with preparations of intestinal nucleoprotein, spleen nuclei, mouse thymus cells, or human kidney T cells whose DNA had been labeled with both [3H]thymidine (TdR) and [125I]-iododeoxyuridine (IUdR). Since free TdR is reutilized more efficiently than free IUdR produced by enzymic hydrolysis of the exogenous DNA, the ratio of [3H]TdR/[125I]IUdR in the DNA fraction of the tissues of the recipient mice provides a measure of the amount of intact exogenous DNA in the tissue. In most instances, the doubly labeled exogenous DNA was almost completely hydrolyzed within 1 day injection, but survival of the DNA from whole cells could be demonstrated in some cases.

References

Jan 1, 1966·Journal of Molecular Biology·K Marushige, J Bonner
Oct 1, 1963·The Journal of Cell Biology·A BENDICHD J HUTCHISON
Apr 1, 1964·The Journal of Cell Biology·K BENSCHL MILLER

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Citations

Jan 1, 1981·European Journal of Nuclear Medicine·L E FeinendegenG Stöcklin
Jan 1, 1982·Annals of Neurology·J S WolinskyO Narayan
Apr 12, 2012·International Journal of Radiation Biology·John M AkuduguRoger W Howell

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