Double long-chain amidine liposome-mediated self replicating RNA transfection

FEBS Letters
A El OuahabiJ M Ruysschaert

Abstract

We present experimental evidence that a complex made of a double long chain cationic amphiphile and recombinant mRNA facilitates the entry and expression of genetic material into cells. Combining the properties of the self replicating recombinant mRNA driven by the Semliki Forest Virus (SFV) replicon and the transfection potentialities of a new cationic amphiphile (N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine) yields a highly efficient mRNA transfection system conferring up to 100% infectivity. The preparation and characterization of the long chain amidine cationic amphiphile-mRNA complex as well as the influence of the diC14-amidine/RNA ratio on the infective activity are described.

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Citations

Aug 11, 1997·Advanced Drug Delivery Reviews·J Zabner
Dec 12, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yuhua WangLeaf Huang
Oct 16, 2014·Biochimica Et Biophysica Acta·Julio H K RozenfeldM Teresa Lamy
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Aug 10, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Julio H K RozenfeldM Teresa Lamy
Feb 16, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Tiago R OliveiraCaroline Lonez
May 24, 2007·Molecular Membrane Biology·Mustapha OualiMichel Vandenbranden
Aug 17, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A El OuahabiM Vandenbranden
Jul 15, 2000·The Journal of Biological Chemistry·V PectorJ M Ruysschaert
Aug 25, 2017·Biophysics Reviews·Julio H K RozenfeldM Teresa Lamy
Apr 13, 2021·Emergent Materials·Thibault ColombaniSidi A Bencherif
Jul 31, 1998·Molecular Genetics and Metabolism·K SawaiD Meruelo
Oct 26, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Geertrui TavernierJoanna Rejman

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