Double null of selenium-glutathione peroxidase-1 and copper, zinc-superoxide dismutase enhances resistance of mouse primary hepatocytes to acetaminophen toxicity.

Experimental Biology and Medicine
Jian-Hong Zhu, Xin Gen Lei

Abstract

This study was conducted to determine the impact of knockout of selenium (Se)-dependent glutathione peroxidase-1 (GPX1-/-) or double knockout of GPX1 and copper, zinc (Cu,Zn)-super-oxide dismutase (SOD1) on cell death induced by acetaminophen (APAP) and its major toxic metabolite N-acetyl-P-benzoquinoneimine (NAPQI). Primary hepatocytes were isolated from GPX1-/-, double knockout of GPX1 and SOD1 (DKO), and their wild-type (WT) mice and were treated with 5 mM APAP or 100 microM NAPQI for 0, 6, and 12 hrs. Compared with the WT cells, the GPX1-/- and DKO hepatocytes were more resistant (P < 0.05) to the APAP-induced cell death but less resistant to the NAPQI-induced cell death. The APAP-mediated glutathione (GSH) depletion was greater (P < 0.05) at 6 hrs in the WT cells than in the GPX1-/- and DKO cells, whereas there was no genotype effect on the NAPQI-mediated GSH depletion. The DKO cells had lower (P < 0.05) microsomal cytochrome P450 2E1 activities, but higher (P < 0.05) glutathione reductase and thioredoxin reductase activities than the WT cells at 0 hrs, and they responded differently to the APAP and NAPQI treatments. Glutathione-S-transferase activity was not affected by genotypes or treatments. Neither APAP nor NAPQI indu...Continue Reading

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Citations

Dec 19, 2015·Physiological Reviews·Xin Gen LeiElias S J Arnér
Nov 26, 2009·Experimental Biology and Medicine·Jian-Hong ZhuXin Gen Lei
Jul 6, 2014·Physiological Reviews·Vyacheslav M LabunskyyVadim N Gladyshev
Jun 26, 2013·Antioxidants & Redox Signaling·Xinhui WangXin Gen Lei
May 1, 2007·Annual Review of Nutrition·Xin Gen LeiJames P McClung
Jun 25, 2008·Free Radical Biology & Medicine·Jian-Hong ZhuXin Gen Lei

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