PMID: 18413066Apr 17, 2008Paper

Double probing of human spermatozoa for persistent histones, surplus cytoplasm, apoptosis and DNA fragmentation

Reproductive Biomedicine Online
Leyla SatiGabor Huszar

Abstract

Individual spermatozoa were assessed with pairs of probes for persistent histones and cytoplasmic retention, persistent histones and DNA fragmentation, and persistent histones and apoptotic markers. The individual spermatozoa were treated sequentially with combinations of probes for these cytoplasmic and nuclear biochemical markers. Sperm fields were recorded with computer-assisted imaging, and staining patterns with the two probes in the same spermatozoa were examined and scored as light, intermediate or dark (mature to arrested-maturity spermatozoa). The effects of arrested sperm maturation were similar with respect to the cytoplasmic and nuclear characteristics of spermatozoa in 84% of cells, indicating that cytoplasmic and nuclear attributes of arrested sperm maturation are related. However, there were moderate (intermediate-dark or intermediate-light patterns, 14.5% of cells) or major (light-dark patterns, 1.6% of cells) discrepancies in the intensity of the double staining patterns. Thus, testing with single maturity markers may not be fully reliable. These findings are important with respect to: (i) arrested sperm maturation; (ii) potential efficacy of antioxidant and similar therapeutic strategies in subfertile men, as ...Continue Reading

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Citations

Sep 22, 2009·Journal of Assisted Reproduction and Genetics·Armand ZiniAbdulaziz Baazeem
Feb 18, 2011·Journal of Assisted Reproduction and Genetics·Armand ZiniNaif Al-Hathal
Aug 29, 2009·Reproductive Biomedicine Online·Stéphanie BellocYves Ménézo
Jan 30, 2010·Asian Journal of Andrology·Rupert P Amann
Aug 4, 2009·Molecular Human Reproduction·R J Aitken, G N De Iuliis
Mar 4, 2014·Reproductive Biomedicine Online·N GeertsG Huszar
Jun 15, 2012·Best Practice & Research. Clinical Obstetrics & Gynaecology·Allan A Pacey
Feb 5, 2009·Reproductive Biomedicine Online·Petra PrinosilovaGabor Huszar
Dec 19, 2015·Journal of Assisted Reproduction and Genetics·Dimitrios IoannouHelen G Tempest
May 21, 2009·Microscopy Research and Technique·Sonja GrunewaldAshok Agarwal
Jun 15, 2010·Fertility and Sterility·Alinne ColinSergio Oehninger
Nov 19, 2017·Journal of Assisted Reproduction and Genetics·Bikem SoygurLeyla Sati
Mar 7, 2018·Environmental Science and Pollution Research International·Ammar OumaimaMehdi Meriem
Jul 19, 2017·Andrology·A S RexJ Fedder

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis