Double-staining of horizontal and amacrine cells by intracellular injection with lucifer yellow and biocytin in carp retina

Neuroscience
T Teranishi, K Negishi

Abstract

Horizontal and amacrine cells in the isolated carp retina were impaled with micropipette electrode, identified by their characteristic light responses, and injected iontophoretically with markers for morphological study. Both Lucifer Yellow CH and biocytin were injected simultaneously. Lucifer Yellow was seen by its own fluorescence while biocytin was visualized by binding with Texas Red-linked or horseradish peroxidase-conjugated avidin. For cone-connected horizontal cells, biocytin-coupled cells were found to be approximately five-times more numerous than Lucifer Yellow-coupled cells. Coupling for both tracers was consistently hampered by intravitreally applied dopamine. In untreated retinas, the injected Lucifer Yellow was restricted within one rod-connected horizontal cell, while biocytin revealed several coupled neighbors. Amacrine cells, labeled by the tracers, were morphologically grouped into eight types, based on our earlier classification. Among them, amacrine cells, belonging to three types (Fnd, Pmb or Pma), were confirmed to be Lucifer Yellow-coupled, and the number of biocytin-coupled cells was more numerous (about 2.5 times) than that of Lucifer Yellow-coupled cells. Most amacrine cells (i.e. Pwd, Fnb and Fna) sh...Continue Reading

References

Jul 17, 1990·Neuroscience Letters·K Negishi, T Teranishi
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E M Lasater
Feb 1, 1988·The Journal of General Physiology·T Saito, T Kujiraoka
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E M Lasater, J E Dowling
Jan 1, 1985·Neuroscience Research. Supplement : the Official Journal of the Japan Neuroscience Society·T TeranishiS Kato
Sep 28, 1984·Neuroscience Letters·T TeranishiS Kato

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Citations

Jul 21, 2007·Visual Neuroscience·W Wade KothmannJohn O'Brien
Sep 24, 2005·Journal of Integrative Neuroscience·Soh HidakaYoko Hashimoto
Jul 14, 1997·The Journal of Comparative Neurology·D Xin, S A Bloomfield

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