PMID: 8598054Mar 1, 1996Paper

Double-strand break-induced mitotic gene conversion: examination of tract polarity and products of multiple recombinational repair events

Current Genetics
Y S WengJac A Nickoloff

Abstract

Double-strand break (DSB)-induced gene conversion in yeast was studied in crosses between ura3 heteroalleles carrying phenotypically silent markers at approximately 100-bp intervals, which allow high-resolution analyses of tract structures. DSBs were introduced in vivo by HO nuclease at sites within shared homology and were repaired using information donated by unbroken alleles. Previous studies with these types of crosses showed that most tracts of Ura+ products are continuous, unidirectional, and extend away from frameshift mutations in donor alleles. Here we demonstrate that biased tract directionality is a consequence of selection pressure against Ura- products that results when frameshift mutations in donor alleles are transferred to recipient alleles. We also performed crosses in which frameshift mutations in recipient and donor alleles were arranged such that events initiated at DSBs could not convert broken alleles to Ura+ via a single gap repair event or a single long-tract mismatch repair event in heteroduplex DNA. This constraint led to low recombination frequencies relative to unconstrained crosses, and inhibited preferential conversion of broken alleles. Physical analysis of 51 DSB-induced products arising from mul...Continue Reading

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Citations

Aug 1, 2000·Mutation Research·J E Haber
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·N SugawaraJ E Haber
Feb 13, 2003·Nucleic Acids Research·Sean PalmerJac A Nickoloff
Jun 21, 2005·Genetics·Scott P KeelyNeil Hall
Oct 13, 2016·Annual Review of Genetics·James E Haber
Oct 6, 2000·Mutation Research·Y S WengJ A Nickoloff
Jun 25, 2015·Microbiology Spectrum·Cheng-Sheng Lee, James E Haber

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