Double-strand break repair by interchromosomal recombination: an in vivo repair mechanism utilized by multiple somatic tissues in mammals

PloS One
Ryan R WhiteChristine Richardson

Abstract

Homologous recombination (HR) is essential for accurate genome duplication and maintenance of genome stability. In eukaryotes, chromosomal double strand breaks (DSBs) are central to HR during specialized developmental programs of meiosis and antigen receptor gene rearrangements, and form at unusual DNA structures and stalled replication forks. DSBs also result from exposure to ionizing radiation, reactive oxygen species, some anti-cancer agents, or inhibitors of topoisomerase II. Literature predicts that repair of such breaks normally will occur by non-homologous end-joining (in G1), intrachromosomal HR (all phases), or sister chromatid HR (in S/G(2)). However, no in vivo model is in place to directly determine the potential for DSB repair in somatic cells of mammals to occur by HR between repeated sequences on heterologs (i.e., interchromosomal HR). To test this, we developed a mouse model with three transgenes-two nonfunctional green fluorescent protein (GFP) transgenes each containing a recognition site for the I-SceI endonuclease, and a tetracycline-inducible I-SceI endonuclease transgene. If interchromosomal HR can be utilized for DSB repair in somatic cells, then I-SceI expression and induction of DSBs within the GFP repo...Continue Reading

References

Jun 1, 1976·The Journal of Experimental Medicine·N Rosenberg, D Baltimore
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H R HwuR J Britten
Jan 1, 1988·Progress in Nucleic Acid Research and Molecular Biology·J F Ward
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R K HumphriesC J Eaves
Nov 1, 1995·Nature Medicine·M Nyström-LahtiB Vogelstein
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·P RouetM Jasin
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B N AmesT M Hagen
Jan 1, 1996·Progress in Nucleic Acid Research and Molecular Biology·C W Schmid
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E Haber, W Y Leung
Jan 1, 1994·Advances in Pharmacology·N OsheroffM J Robinson
Jan 24, 1997·Cell·R ScullyD M Livingston
May 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·K SmallS T Warren
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·M E Moynahan, M Jasin
Aug 8, 1997·The Journal of Biological Chemistry·K B Beckman, B N Ames
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·F LiangM Jasin
Oct 21, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C KollmannsbergerC Bokemeyer
Dec 23, 1998·Annals of Oncology : Official Journal of the European Society for Medical Oncology·F CelsingM Björkholm
Dec 29, 1998·Mechanisms of Development·A K HadjantonakisA Nagy
Jul 3, 1999·Trends in Biochemical Sciences·J E Haber
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·R StrickJ D Rowley
Jul 19, 2000·The Plant Cell·G BlancM Delseny
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M E DolléJ Vijg
Mar 21, 2001·Nature Reviews. Genetics·D C van GentR Kanaar
Apr 3, 2001·Nature Reviews. Genetics·T Cremer, C Cremer
Mar 28, 2002·Cellular and Molecular Life Sciences : CMLS·B Elliott, M Jasin
May 29, 2002·Oncogene·Joseph M ScanduraStephen D Nimer
Aug 31, 2002·Genes, Chromosomes & Cancer·Elena KolomietzJeremy A Squire
Dec 10, 2002·Mechanisms of Ageing and Development·Tetsuya OnoHironobu Ikehata
Jan 2, 2003·Molecular and Cellular Biology·Jeremy M Stark, Maria Jasin
May 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Carrie A HendricksBevin P Engelward
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·C Richardson, M Jasin
May 27, 2003·Nature Cell Biology·Michael LisbyRodney Rothstein
Aug 5, 2003·Molecular and Cellular Biology·Kai RothkammMarkus Löbrich
Oct 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michael M Vilenchik, Alfred G Knudson

❮ Previous
Next ❯

Citations

May 16, 2014·Nature Reviews. Molecular Cell Biology·Jörg RenkawitzStefan Jentsch
Jun 9, 2016·Human Genetics·Daniel StoneKeith R Jerome
Jan 21, 2017·Trends in Genetics : TIG·Nuno AmaralIrene Chiolo
Dec 22, 2016·Aging Cell·Laetitia DelabaereJeannine R LaRocque
Aug 30, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P Christopher CaridiIrene Chiolo
Sep 5, 2019·Nature Cell Biology·Christopher Patrick CaridiIrene Chiolo
Dec 14, 2016·Aging·Margarita PustovalovaAndreyan N Osipov
Sep 23, 2021·GeroScience·Michelle L SwiftJane Azizkhan-Clifford

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transgenic
activated cell sorting
FACS
genotyping

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cancer Epigenetics and Chromatin (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on chromatin and its role in cancer epigenetics please follow this feed to learn more.

Cancer Epigenetics Chromatin Complexes (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on chromatin complexes and their role in cancer epigenetics.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.