PMID: 7024323Sep 1, 1981Paper

Double-stranded DNA antibodies: a comparison of four methods of detection

Journal of Clinical Pathology
S D SomerfieldR J Booth

Abstract

Thirty-four antinuclear antibody (ANA) positive systemic lupus erythematosus (SLE) sera were tested for antibodies to double-stranded DNA (dsDNA) simultaneously using Farr, haemagglutination, Crithidia luciliae (CL) kinetoplast fluorescence and human metaphase chromosome fluorescence assays. Significant correlation (p less than 0 x 05) was found between the Farr and CL assays, with the two fluorescence tests (CL and metaphase) displaying the greatest degree of association (p = 0 x 00001). No correlation could be demonstrated between the haemagglutination test and any of the other three assays. Six hundred and ninety-one sera from patients with a range of provisional rheumatological diagnoses were prospectively analysed for dsDNA antibodies using Farr and metaphase assays. A correlation coefficient of 0 x 84 was obtained between the two assays. The metaphase assay provides comparable results to other more established assays, and because it is simple, reliable and sensitive, it should be seriously considered for routine use in testing for dsDNA antibodies.

Citations

Oct 17, 2002·Arthritis and Rheumatism·Arthur F KavanaughUNKNOWN American College of Rheumatology Ad Hoc Committee on Immunologic Testing Guidelines
Jun 1, 1987·Annals of the Rheumatic Diseases·D A IsenbergA Todd-Pokropek
Nov 1, 1987·Journal of Clinical Pathology·D A Isenberg, P J Maddison
Feb 28, 2016·Journal of Immunological Methods·Gabriella LakosThierry Dervieux
Jan 1, 1985·Scandinavian Journal of Rheumatology·T K Lee, H Rothberger
May 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L E GurianJ N Gilliam
Sep 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J R WoodH Markowitz
Apr 3, 2019·Experimental and Therapeutic Medicine·Yi YuanZhenyu Jiang

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