PMID: 9535222Apr 16, 1998Paper

Double-stranded DNA can be translocated across a planar membrane containing purified mitochondrial porin

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
I SzabòM Zoratti

Abstract

The transport of genetic material across biomembranes is a process of great relevance for several fields of study. However, much remains to be learned about the mechanisms underlying transport, one of which implies the involvement of proteic DNA-conducting pores. Entry of genetic material into mitochondria has been observed under both physiological and pathological conditions. We report here that double-stranded DNA can move through a planar bilayer membrane containing isolated mitochondrial porin (voltage-dependent anion channel). The transport is driven by the applied electrical field, and the presence of DNA is associated with a decrease of current conduction by the pores. The passage of DNA does not take place if the bilayer has not been doped with any protein or in the presence of both reconstituted porin and anti-porin antibody. Translocation does not occur if the bilayer contains Shigella sonnei maltoporin, gramicidin A channels, or a 30 pS anion-selective channel plus other proteins. These results show that mitochondrial porin is capable of mediating the transport of genetic material, revealing a new property of this molecule and further confirming the idea that DNA can move through proteic pores.

References

Jan 27, 1978·Science·R M Schwartz, M O Dayhoff
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M W McEneryS H Snyder
Feb 1, 1992·Journal of Bioenergetics and Biomembranes·C A MannellaM Colombini
Feb 1, 1992·Journal of Bioenergetics and Biomembranes·F P Thinnes
May 3, 1991·Cell·S M Simon, G Blobel
Jun 15, 1990·Cell·E S Lander, H Lodish
Nov 3, 1989·Biochimica Et Biophysica Acta·G Glaser-WuttkeI Rasched
Dec 11, 1987·Biochimica Et Biophysica Acta·V de PintoF Palmieri
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·R DermietzelD C Spray
Jul 28, 1994·Nature·S M BezrukovV A Parsegian
Oct 1, 1994·Journal of Bioenergetics and Biomembranes·M Zoratti, I Szabó
May 24, 1995·Biochimica Et Biophysica Acta·D C WallaceM T Lott
Jul 17, 1995·Biochimica Et Biophysica Acta·M Zoratti, I Szabò
Jun 1, 1994·Journal of Bioenergetics and Biomembranes·M D Brown, D C Wallace
Sep 1, 1994·The Journal of Cell Biology·M SomasundaranR H Singer
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·P ZamecnikG Witman
Apr 29, 1994·Biochemical and Biophysical Research Communications·G Rauch, O Moran
Jan 1, 1993·Annual Review of Microbiology·V Citovsky, P Zambryski
Oct 1, 1995·The Journal of Membrane Biology·Y E KorchevC A Pasternak
Aug 1, 1995·The Journal of Membrane Biology·J O BustamanteA Liepins
Jan 1, 1996·Ion Channels·M ColombiniM Forte
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J KasianowiczD W Deamer
Mar 7, 1997·Journal of Molecular Biology·J E MeyerG E Schulz
Apr 1, 1996·Journal of Bioenergetics and Biomembranes·J Song, M Colombini
Oct 6, 1997·The Journal of Biological Chemistry·I SzabòM Zoratti

❮ Previous
Next ❯

Citations

Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·S HoworkaH Bayley
Aug 11, 2006·American Journal of Physiology. Cell Physiology·Shaharyar M KhanRussell H Swerdlow
Aug 25, 2007·Nanomedicine·Ken Healy
Jul 19, 2008·Annual Review of Analytical Chemistry·John J KasianowiczVincent M Stanford
Mar 1, 2012·PLoS Neglected Tropical Diseases·Irene ZornettaCesare Montecucco
Dec 3, 2005·Human Molecular Genetics·Milana KoulintchenkoRobert N Lightowlers
Aug 24, 2005·Nucleic Acids Research·Hwa DaiKwen-Sheng Chiang
Nov 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Thalia SalinasLaurence Maréchal-Drouard
Jun 2, 2011·Biological Chemistry·Marco LolicatoVito De Pinto
May 13, 2006·The Journal of Chemical Physics·Yongjun XieJie Chen
Jan 7, 2011·American Journal of Physiology. Cell Physiology·Boris F KrasnikovDmitry B Zorov
Apr 3, 2014·Physiological Reviews·Ildiko Szabo, Mario Zoratti
Apr 24, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Vanessa Checchetto, Ildiko Szabo
May 6, 2016·Journal of Bioenergetics and Biomembranes·María Luisa CampoSonia Martínez-Caballero
Apr 28, 2020·Critical Reviews in Biotechnology·Poonam KanwarGirdhar K Pandey
Aug 12, 2003·Journal of Neurotrauma·Jose L Perez VelazquezMarina V Frantseva
Dec 15, 2015·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Steve PerryR W M Kwong
May 8, 2016·Nature Biotechnology·John J Kasianowicz, Sergey M Bezrukov
Feb 12, 2021·International Journal of Molecular Sciences·Carmen A Mannella
Mar 15, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Perla Pérez-TreviñoNoemí García
Jan 31, 2015·Molecular Pharmaceutics·Ana M CardosoAmália S Jurado
Aug 28, 2021·Clinical Transplantation·Changzhen HaoWei Wang
Dec 14, 2011·Biochimica Et Biophysica Acta·Fabrice HombléMartine Prévost

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Conduction System

The cardiac conduction system is a specialized tract of myocardial cells responsible for maintaining normal cardiac rhythm. Discover the latest research on the cardiac conduction system here.