Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Journal of Visualized Experiments : JoVE
Saikat Kumar B GhoshDawn E Gundersen-Rindal

Abstract

Phloem and plant sap feeding insects invade the integrity of crops and fruits to retrieve nutrients, in the process damaging food crops. Hemipteran insects account for a number of economically substantial pests of plants that cause damage to crops by feeding on phloem sap. The brown marmorated stink bug (BMSB), Halyomorpha halys (Heteroptera: Pentatomidae) and the Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae) are hemipteran insect pests introduced in North America, where they are an invasive agricultural pest of high-value specialty, row, and staple crops and citrus fruits, as well as a nuisance pest when they aggregate indoors. Insecticide resistance in many species has led to the development of alternate methods of pest management strategies. Double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) is a gene silencing mechanism for functional genomic studies that has potential applications as a tool for the management of insect pests. Exogenously synthesized dsRNA or small interfering RNA (siRNA) can trigger highly efficient gene silencing through the degradation of endogenous RNA, which is homologous to that presented. Effective and environmental use of RNAi as molecular biopesticides for bi...Continue Reading

Citations

May 11, 2019·International Journal of Molecular Sciences·Alexandra S Dubrovina, Konstantin V Kiselev
Aug 17, 2019·Pest Management Science·Nitish KunteLuz-Adriana Avila
Nov 22, 2019·Neotropical Entomology·N P DiasM J Zotti
Jul 23, 2020·Molecular Biology Reports·Olawale Samuel AdeyinkaBushra Tabassum
Oct 28, 2019·International Journal of Molecular Sciences·Lifang SunJianguo Xu
Jun 16, 2021·Frontiers in Plant Science·Demetrio MarcianòSilvia Laura Toffolatti
May 5, 2021·Plant Biotechnology Journal·Gaetano GiudiceWalter Chitarra

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