Double-vein jugular/inferior vena cava clamp technique for long-term in vivo studies in rats

Physiology & Behavior
R BurvinE Karnieli

Abstract

We present a step-by-step manual for chronic cannulation of rats using a simple technique. This concept facilitates repeated clamping of the same rat over a 6-10-week period, providing a completely separate infusion route from blood sampling access which is placed into mixed venous blood in the inferior vena cava. Permanent catheters implanted into the left external jugular vein and the inferior vena cava were used for miniature blood sampling and recycling. The design and running of clamp experiments and other physiological research models are detailed. Long-term reliable venous access, simple installation, and easy after-care of the rats' cannulas are the principal advantages of the procedure described.

References

May 1, 1987·The Journal of Clinical Investigation·L RossettiR A DeFronzo
May 1, 1973·The Journal of Clinical Investigation·A I Katz, A H Rubenstein
Jun 1, 1974·Hormones and Behavior·J Terkel, L Urbach
Jul 1, 1994·Physiology & Behavior·R BurvinE Karnieli
Sep 13, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·R BurvinE Karnieli

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Citations

Jul 6, 2011·Journal of Neuroscience Methods·Patrick C HettingerJames S Hyde
Dec 5, 2006·The Journal of Nutritional Biochemistry·Clemence BlouetJean-François Huneau
Jun 19, 2016·Thrombosis Research·Christi M TerryAlfred K Cheung
Aug 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Jean-Christophe J BouthegourdPatrick C Even

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