Doublet cells in Tetrahymena as indicators of culture media composition

Biological Trace Element Research
G K Christopher, C A Sundermann

Abstract

Stomatogenesis in ciliates is a complex and carefully orchestrated event. The exo- mutant SB255 of Tetrahymena thermophila has defects in mucocyst formation and docking and can also have one or two mouths. Three common culture media (proteose peptone, Medium 357, and yeast extract) were analyzed for total C, N, and inorganic elements and then tested for their effect on the number of mouths present in SB255. Cultures of SB255 grown in Medium 357 consisted of a mixed population of cells with either two mouths (doublet) or one mouth. Cultures from the same original stock grown in Medium 357 (SBm) and in 1% proteose peptone (SBpp) had different percentages of doublet cells in 1-, 2-, 3-, and 4-d-old cultures. When transferred to and grown in 1% yeast medium, both SBpp and SBm cultures had increased percentages of doublets over a 4-d culture period. When grown in 0.1, 0.5, or 1% yeast medium for 2 d, both SBpp and SBm cultures had more doublets in 1% than in either 0.1 or 0.5% yeast medium. Cultures of SBm grown in Medium 357 or 1% yeast medium for 2 d had a 10-fold increase in doublet cells compared to the inoculum. After 2 d in 1% proteose peptone, SBm cultures had percentages of doublet cells almost equal to that of the inoculum....Continue Reading

References

Sep 30, 1992·Annals of the New York Academy of Sciences·V V AgteM K Gokhale
Jun 5, 1987·Science·W J Gehring
Aug 9, 1974·Nature·M P Capdevila, A Garcia-Bellido
Jul 15, 1967·Nature·D T Saggers, M L Clark
Jan 1, 1951·Physiological Zoology·G W KIDDERR E PARKS

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