Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells

World Journal of Gastroenterology : WJG
Yi-Feng WuKai-Xian Qian

Abstract

To investigate the effect of schisandrin B (Sch B) on proliferation and apoptosis of human hepatoma SMMC-7721 cells in vitro and regulation of Hsp70 and Caspases-3, 7, 9 expression by Sch B. Human hepatoma cell line SMMC-7721 was cultured and treated with Sch B at various concentrations. Growth suppression was detected with MTT colorimetric assay. Cell apoptosis was confirmed by DNA ladder detection and flow cytometric analysis. The expression of Hsp70, Caspases-3, 7, 9 were analyzed by Western blot analysis. Sch B inhibited the growth of hepatoma SMMC-7721 cells in a dose-dependent manner, leading to a 50% decrease in cell number (LC50) value of 23.50 mg/L. Treatment with Sch B resulted in degradation of chromosomal DNA into small internucleosomal fragments, evidenced by the formation of a 180-200 bp DNA ladder on agarose gels. FCM analysis showed the peak areas of subdiploid at the increased concentration of Sch B. The results of Western bolt analysis showed that Hsp70 was down-regulated and Caspase-3 was up-regulated, while the activity of Caspases-7, -9 had no significant change. Sch B is able to inhibit the proliferation of human hepatoma SMMC-7721 cells and induce apoptosis, which goes through Caspase-3-dependent and Casp...Continue Reading

References

Jan 1, 1991·Memórias do Instituto Oswaldo Cruz·X Y Li
Feb 25, 1996·Experimental Cell Research·A Samali, T G Cotter
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Nov 3, 1998·The EMBO Journal·M JäätteläM Egeblad
Jun 15, 2000·Experimental Cell Research·E M CreaghT G Cotter
Jun 19, 2001·International Journal of Cancer. Journal International Du Cancer·U Törmänen-NäpänkangasP Pääkkö
May 7, 2002·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·J Held, K Schulze-Osthoff
Sep 11, 2002·Apoptosis : an International Journal on Programmed Cell Death·H WajantP Scheurich
Sep 5, 2002·Free Radical Biology & Medicine·Carmen Díez-FernándezMaría Cascales
Sep 10, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hidekazu InoueTakeshi Nakano
Dec 20, 2002·American Journal of Physiology. Cell Physiology·Tom S LiuEugene B Chang
Apr 11, 2003·Medical Microbiology and Immunology·Paula C S SoutoLiana Verinaud
Sep 2, 2003·Methods and Findings in Experimental and Clinical Pharmacology·J-H LiuR-Y Wu
Dec 4, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·M EriguchiM Sekiguchi
Dec 10, 2003·The Lancet Oncology·Wei Hu, John J Kavanagh

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Citations

Oct 6, 2007·The International Journal of Neuroscience·Shuqiao YaoXiaoli Qi
Apr 8, 2020·Oxidative Medicine and Cellular Longevity·M I NasserPing Zhu
Jun 6, 2012·Oxidative Medicine and Cellular Longevity·Philip Y Lam, Kam Ming Ko
Aug 4, 2016·Chinese Journal of Integrative Medicine·Ling QiYe Xu
Sep 8, 2011·Clinical and Experimental Pharmacology & Physiology·Pou K LeongKam M Ko
Nov 17, 2016·Oxidative Medicine and Cellular Longevity·Pou Kuan Leong, Kam Ming Ko
Jun 24, 2021·PLoS Neglected Tropical Diseases·Ho Yin Pekkle LamShih-Yi Peng
Dec 26, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jong-Hwan KimYung Hyun Choi

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