Down-regulated expression of Annexin A7 induces apoptosis in mouse hepatocarcinoma cell line by the intrinsic mitochondrial pathway

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Yuhong HuangJianwu Tang

Abstract

Our previous studies have shown that decreased expression of Annexin A7 elevates apoptosis in Hca-P cells, a hepatocarcinoma cell line with lymphatic metastatic potential. In this study, RNA interference technique was used to down-regulate the expression of Annexin A7, and unmanipulated Hca-P cells and transfected nonspecific-sequence Hca-P cells as control. The down-regulation of Annexin A7 declined the cell viability after cisplatin exposure. And the reduced expression of Annexin A7 decreased the expression of Bcl2, increased the expression of Cytochrome-C in the cytoplasme, and then improved the expression of Caspase-3. However there was no significant effect on the expression of Bax, Caspase-12, Fas, FasL and Caspase-8. The results indicate that the decreased expression of Annexin A7 could inhibit the proliferation, and increase the apoptosis of Hca-P cells by affecting the expression of the apoptosis associated proteins by the mitochondrial pathway.

References

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Apr 2, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yan-Ling JinJian-Wu Tang
Jan 1, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lin SongJian-Wu Tang
Feb 18, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xianyan WangJianwu Tang
Jul 30, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuhong HuangJianwu Tang

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Citations

Aug 11, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xian-Yan WangJian-Wu Tang
Oct 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lulu BaiJianwu Tang
Jun 13, 2018·Journal of Cellular Biochemistry·Yuanli HuangYuanrong Yang
Aug 26, 2019·American Journal of Reproductive Immunology : AJRI·Hui-Qin MoYan Zhang

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