Down-regulating IL-6/GP130 targets improved the anti-tumor effects of 5-fluorouracil in colon cancer

Apoptosis : an International Journal on Programmed Cell Death
Sanhong LiZhenling Ji

Abstract

Recent studies have confirmed that IL-6/GP130 targets are closely associated with tumor growth, metastasis and drug resistance. 5-Fluorouracil (5-FU) is the most common chemotherapeutic agent for colon cancer but is limited due to chemoresistance and high cytotoxicity. Bazedoxifene (BZA), a third-generation selective estrogen receptor modulator, was discovered by multiple ligand simultaneous docking and drug repositioning approaches to have a novel function as an IL-6/GP130 target inhibitor. Thus, we speculated that in colon cancer, the anti-tumor efficacy of 5-FU might be increased in combination with IL-6/GP130 inhibitors. CCK8 assay and colony formation assay were used to detect the cell proliferation and colony formation. We measured the IC50 value of 5-FU alone and in combination with BZA by cell viability inhibition. Cell migration and invasion ability were tested by scratch migration assays and transwell invasion assays. Flow cytometric analysis for cell apoptosis and cell cycle. Quantitative real-time PCR was used to detect Bad, Bcl-2 and Ki-67 mRNA expression and western blotting (WB) assay analyzed protein expression of Bad/Bcl-2 signaling pathway. Further mechanism study, WB analysis detected the key proteins level i...Continue Reading

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Citations

Mar 9, 2020·Apoptosis : an International Journal on Programmed Cell Death·Patrycja Nowak-Sliwinska, Arjan W Griffioen
Dec 26, 2018·Expert Opinion on Therapeutic Targets·Camila Florencia ZuccatoMarianela Candolfi
May 24, 2019·International Journal of Molecular Sciences·Iris C SalaroglioChiara Riganti
Jun 12, 2020·Cancer Letters·Rui HuJian Zhang
Apr 20, 2019·Journal of Medicinal Chemistry·Ran ZhangJing-Yuan Xu
Aug 17, 2021·Journal of Biochemical and Molecular Toxicology·Dongxin TangGang Cao
Jul 4, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Debasish Kumar DeySun Chul Kang

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Methods Mentioned

BETA
surgical resection
xenograft
PCR
xenografts
FACS
flow cytometry

Software Mentioned

ModFit LT
GraphPad Prism
Cell Quest

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