Down Regulation and Loss of Auxin Response Factor 4 Function Using CRISPR/Cas9 Alters Plant Growth, Stomatal Function and Improves Tomato Tolerance to Salinity and Osmotic Stress.

Genes
Sarah BouzroudMohamed Zouine

Abstract

Auxin controls multiple aspects of plant growth and development. However, its role in stress responses remains poorly understood. Auxin acts on the transcriptional regulation of target genes, mainly through Auxin Response Factors (ARF). This study focuses on the involvement of SlARF4 in tomato tolerance to salinity and osmotic stress. Using a reverse genetic approach, we found that the antisense down-regulation of SlARF4 promotes root development and density, increases soluble sugars content and maintains chlorophyll content at high levels under stress conditions. Furthermore, ARF4-as displayed higher tolerance to salt and osmotic stress through reduced stomatal conductance coupled with increased leaf relative water content and Abscisic acid (ABA) content under normal and stressful conditions. This increase in ABA content was correlated with the activation of ABA biosynthesis genes and the repression of ABA catabolism genes. Cu/ZnSOD and mdhar genes were up-regulated in ARF4-as plants which can result in a better tolerance to salt and osmotic stress. A CRISPR/Cas9 induced SlARF4 mutant showed similar growth and stomatal responses as ARF4-as plants, which suggest that arf4-cr can tolerate salt and osmotic stresses. Our data supp...Continue Reading

References

Dec 1, 1971·The Biochemical Journal·W J Broughton, M J Dilworth
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·A BurbidgeI B Taylor
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Paul M. HasegawaHans J. Bohnert
Mar 3, 2006·Journal of Experimental Botany·Rana MunnsAndré Läuchli
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Taishi UmezawaKazuo Shinozaki
Feb 1, 1974·Plant Physiology·R E Smart
Sep 29, 2007·Current Opinion in Plant Biology·Tom J Guilfoyle, Gretchen Hagen
Mar 24, 2009·Cell·Steffen Vanneste, Jirí Friml
Mar 27, 2009·Journal of Experimental Botany·Maaike de JongWim H Vriezen
May 7, 2010·Journal of Biomedicine & Biotechnology·Valérie JaulneauBernard Dumas
May 26, 2010·Functional & Integrative Genomics·SuiKang WangYanHua Qi
Jun 16, 2010·Plant, Cell & Environment·Gaëlle DamourLaurent Urban
Nov 29, 2011·Plant Science : an International Journal of Experimental Plant Biology·Asim KadiogluAykut Saglam
Mar 23, 2012·Physiologia Plantarum·Abidur Rahman
Apr 3, 2012·Journal of Experimental Botany·Nicky J Atkinson, Peter E Urwin
May 23, 2012·Plant Physiology and Biochemistry : PPB·Franklin Aragão GondimJosé Tarquinio Prisco
Feb 22, 2013·Plant Signaling & Behavior·Shuvasish ChoudhurySanjib Kumar Panda
Jul 3, 2013·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Chien Van HaLam-Son Phan Tran
Nov 10, 2013·Frontiers in Plant Science·Louise H ComasDavid A Dierig
Feb 18, 2014·Plant Physiology and Biochemistry : PPB·Rongchao YangYang-Dong Guo
Feb 13, 2016·Frontiers in Plant Science·Si-Bei LiJin-Zhi Zhang
Feb 9, 2017·Plant Science : an International Journal of Experimental Plant Biology·Agustin ZsögönLázaro Eustáquio Pereira Peres

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Citations

May 21, 2020·Journal of Experimental Botany·Tomas RibbaJosé Antonio O'Brien
Apr 4, 2021·International Journal of Molecular Sciences·Mengyi ChenYanwei Hao
Apr 13, 2021·Journal of Advanced Research·Dangquan ZhangBaohong Zhang
Apr 10, 2021·Journal of Zhejiang University. Science. B·Chao LiBaohong Zhang
Jul 22, 2021·Journal of Agricultural and Food Chemistry·Shakeel AhmadShaoqing Tang
Sep 3, 2021·International Journal of Biological Macromolecules·Murugesan ChandrasekaranManivannan Paramasivan

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Methods Mentioned

BETA
transgenic
environmental stresses
genotyping
PCR
salt treatment

Software Mentioned

ContigExpress
Pro Plus
Image
ImageJ

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