PMID: 2502396Jul 1, 1989Paper

Down-regulation of alpha 3(VI) chain expression by gamma-interferon decreases synthesis and deposition of collagen type VI

European Journal of Biochemistry
M HeckmannT Krieg

Abstract

Treatment of cultured human skin fibroblasts with increasing doses of gamma-interferon produces a distinct reduction of steady-state levels of the alpha 3 chain of collagen VI mRNA by about 60% but not of the alpha 1 and alpha 2 chain mRNAs. A similar decrease was also observed for collagen I and III mRNA while fibronectin mRNA remained at the same level. The decrease in alpha 3(VI) mRNA is accompanied by a reduced synthesis of collagen VI and by a reduced deposition of both collagen VI and fibronectin in urea-insoluble form in the cell matrix. No other gamma-interferon effects were observed for fibronectin biosynthesis. Immunoprecipitation of metabolically labeled collagen VI demonstrated a strongly reduced synthesis (by 65-80%) of intracellular alpha 3(VI) chains with no decrease found for alpha 1(VI) and alpha 2(VI) chains. All three chains were, however, found to be reduced in the culture medium. Pepsin treatment of immunoprecipitated collagen VI showed similar chain ratios for material in the culture medium obtained in the absence or presence of gamma-interferon. It indicates that correctly assembled heterotrimers of the composition [alpha 1(VI) alpha 2(VI) alpha 3(VI)] are formed and secreted also in the absence of an equ...Continue Reading

References

Feb 1, 1987·Journal of Cellular Physiology·B C Adelmann-GrillT Krieg
Jul 15, 1988·The Biochemical Journal·T SchreierB Trüeb
Mar 1, 1986·The Journal of Cell Biology·E EngvallG Klier
Mar 1, 1985·The Journal of Clinical Investigation·D J Prockop
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
May 1, 1983·The Biochemical Journal·H FurthmayrJ Engel
Aug 1, 1983·The Journal of Cell Biology·P J McKeown-Longo, D F Mosher
Aug 1, 1984·European Journal of Biochemistry·H von der MarkR Timpl
Aug 30, 1984·Biochemical and Biophysical Research Communications·J RosenbloomS A Jimenez
May 1, 1981·The Journal of Investigative Dermatology·R FleischmajerR Timpl

❮ Previous
Next ❯

Citations

Jul 1, 1991·Journal of Dermatological Science·B Lankat-ButtgereitT Krieg
Feb 1, 1996·Journal of Dermatological Science·A HatamochiH Yoshioka
May 16, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Marie DziadekMon-Li Chu
Jan 1, 1997·The International Journal of Biochemistry & Cell Biology·A Diaz, S A Jiménez
Mar 1, 1992·The British Journal of Dermatology·A HatamochiH Ueki
Sep 15, 1992·European Journal of Biochemistry·E Koller, B Trueb
Oct 15, 1992·European Journal of Biochemistry·A ColombattiF Bucciotti
Dec 21, 2005·Journal of Dermatological Science·Mitsuhito OtaHiroshi Shimizu
Apr 4, 1997·The Journal of Biological Chemistry·B GayraudL Bruckner-Tuderman
Apr 12, 2001·The British Journal of Dermatology·R E WatsonC E Griffiths
Feb 1, 1990·The Journal of Dermatology·T Krieg, M Aumailley
Apr 13, 2000·International Journal of Cancer. Journal International Du Cancer·B Trueb, B F Odermatt
Jun 1, 1993·The Journal of Investigative Dermatology·A HatamochiH Yoshioka
Jan 1, 1993·The Journal of Investigative Dermatology·C MauchT Krieg
Mar 1, 1993·The Journal of Investigative Dermatology·T OonoT Krieg
Jul 1, 1990·Matrix : Collagen and Related Research·P BonaldoA Colombatti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.