Abstract
This study was designed to investigate the p38 mitogen-activated protein kinase (MAPK) signaling pathway involved in Aquaporin1 (AQP1) expression caused by staphylococcal peptidoglycan (PGN) in cultured rat pleural mesothelial cells (rPMCs) in vitro. RT-PCR and immunoblot analysis were used to determine the relative mRNA and protein levels of AQP1 by PGN in rPMCs. P38 kinase inhibitor SB203580, JNK inhibitor SP600125, and ERK1/2 inhibitor PD98059 were used to determine the effects of PGN-induced AQP1 expression by immunoblot. Activation of p38 by PGN was reflected by detecting the phosphorylation constituent of p38, using immunoblot. The shift of localization after activation of p38 by PGN was investigated by immunofluorescence assay. AQP1 transcription and protein expression were decreased by PGN in dose-dependent and time-dependent manners in rPMCs. Down-regulation of AQP1 by PGN was blocked only by SB203580, neither by SP600125 nor by PD98059. Furthermore, rPMCs exposed to PGN showed activation of p38 MAPK. Phospho-p38 protein production was increased by PGN stimulation in rPMCs. The localization of phospho-p38 was both in the cytosol and nuclei after PGN treatment, while its normal distribution is mainly in the cytosol in r...Continue Reading
References
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S NielsenP Agre
Oct 1, 1996·The Journal of Infectious Diseases·R DziarskiD Gupta
Mar 26, 2002·Nature Immunology·Adeline M HajjarSamuel I Miller
Dec 13, 2002·Gut·D W HommesS J H van Deventer
Oct 3, 2003·Cellular and Molecular Life Sciences : CMLS·R Dziarski
Feb 26, 2004·Respirology : Official Journal of the Asian Pacific Society of Respirology·Chadi A HageVeena B Antony
May 15, 2004·American Journal of Respiratory Cell and Molecular Biology·Yasuhiro GonTakashi Horie
Jul 24, 2004·Journal of Medical Microbiology·Clett ErridgeIan R Poxton
Apr 9, 2005·Nature·Samira SaadounA S Verkman
Oct 4, 2005·Biochimica Et Biophysica Acta·Bozena Kaminska
Jan 13, 2006·The Journal of Biological Chemistry·Gang LuYibin Wang
Apr 6, 2007·Journal of Neuroscience Research·Hiroshi YatsushigeJohn H Zhang
May 25, 2007·The Journal of Biological Chemistry·Wei ZhangVedat Schwenger
Sep 19, 2007·Lung·Hongchun DuXiaohua Deng
Nov 13, 2007·Molecular Immunology·In Su CheonSeung Hyun Han
Mar 12, 2008·Respiration; International Review of Thoracic Diseases·Michael A Jantz, Veena B Antony
May 17, 2008·Expert Reviews in Molecular Medicine·A S Verkman
Jul 16, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tajamul HussainKamal A Mohammed
Oct 7, 2009·PloS One·Eloïse RaoustMichel Chignard
Jun 8, 2011·Lung·Lihua Liu, Canmao Xie
Sep 3, 2011·American Journal of Respiratory Cell and Molecular Biology·Sylwia WilkoszY C Gary Lee
Dec 4, 2012·Molecular Immunology·Rituparna ChakrabortyAjit Sodhi
Dec 6, 2012·Investigative Ophthalmology & Visual Science·Liang LiangPeizeng Yang
Citations
Jan 14, 2016·PloS One·Wei ZhangVedat Schwenger
Jul 28, 2016·International Journal of Molecular Sciences·Yiwei ZhangAizhong Wang
Sep 19, 2019·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Bohui LiYingyu Jin
Jun 5, 2017·BMC Complementary and Alternative Medicine·Xin KangGuo-Hui Liu
Nov 9, 2019·Scientific Reports·Lei ZhuQing-Jia Xu
Oct 7, 2020·Medical Hypotheses·Lezy Flora Mariajoseph-AntonyChidambaram Prahalathan