Down-regulation of Bcl-2 and Akt induced by combination of photoactivated hypericin and genistein in human breast cancer cells

Journal of Photochemistry and Photobiology. B, Biology
Peter FerencPeter Fedorocko

Abstract

Presented experiment considers combination of genistein and photodynamic therapy with hypericin with a view to achieve higher therapeutic outcome in human breast adenocarcinoma cell lines MCF-7 and MDA-MB-231, both identified in our conditions as photodynamic therapy resistant. Since genistein is known to suppress Bcl-2 expression, we predicted that photodynamic therapy with hypericin might benefit from mutual therapeutic combination. In line with our expectations, combined treatment led to down-regulation of Bcl-2 and up-regulation of Bax in both cell lines as well as to suppression of Akt and Erk1/2 phosphorylation induced by photoactivated hypericin in MCF-7 cells. Although Akt and Erk1/2 phosphorylation was not stimulated by photodynamic therapy with hypericin in MDA-MB-231 cells, it was effectively suppressed in combination. Variations in cell death signaling favoring apoptosis were indeed accompanied by cell cycle arrest in G(2)/M-phase, activation of caspase-7, PARP cleavage and increased occurrence of cells with apoptotic morphology of nucleus. All these events corresponded with suppression of proliferation and significantly lowered clonogenic ability of treated cells. In conclusion, our results indicate that pre-treatm...Continue Reading

References

May 26, 1995·Biochemical Pharmacology·P AgostinisP De Witte
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M C PagliacciI Nicoletti
Apr 1, 1997·Journal of Photochemistry and Photobiology. B, Biology·A L VandenbogaerdeP A de Witte
May 23, 1998·The Journal of Biological Chemistry·R U JänickeA G Porter
Sep 12, 1998·Science·J M Adams, S Cory
Nov 13, 1998·Science·M H CardoneJ C Reed
Feb 19, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A I ConstantinouJ S Murley
Mar 4, 2000·The Biochemical Journal·B Vanhaesebroeck, D R Alessi
Feb 15, 2001·Endocrine-related Cancer·R KumarL Adam
Feb 15, 2001·Nature Cell Biology·S P TzungD M Hockenbery
Feb 24, 2001·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·A MirossayL Mirossay
Jul 4, 2002·Canadian Journal of Gastroenterology = Journal Canadien De Gastroenterologie·Brian C Wilson
Jul 27, 2002·Planta medica·Peter SolárPeter Fedorocko
Sep 6, 2002·Biochemical Pharmacology·Alok C Bharti, Bharat B Aggarwal
Nov 25, 2003·Photochemistry and Photobiology·Shougang Zhuang, Irene E Kochevar
Sep 30, 2004·Free Radical Research·Stefan M SchiekeLars-Oliver Klotz
Jan 26, 2005·International Journal of Radiation Oncology, Biology, Physics·Takamitsu HaraTomio Inoue
Jun 3, 2006·Photochemistry and Photobiology·Veronika SackováJán Kleban
Sep 22, 2006·International Journal of Cancer. Journal International Du Cancer·Arabinda DasSwapan K Ray
Jun 15, 2007·Surgery·Christina L RolandCarlton C Barnett
Jul 5, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jaromír MikesPeter Fedorocko
Sep 6, 2007·International Journal of Oncology·Chaitali TophkhaneXiaohe Yang
Sep 21, 2007·Photochemistry and Photobiology·Ozguncem BozkulakCharles J Gomer
Sep 28, 2007·Investigative Ophthalmology & Visual Science·Akihisa MatsubaraJoan W Miller
Jan 9, 2008·Photochemistry and Photobiology·David Kessel
Aug 9, 2008·Molecular Cancer Therapeutics·Peter SolárArthur J Sytkowski
Sep 2, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Zhong LiJida Xu

❮ Previous
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Citations

Aug 24, 2010·Cancer Metastasis Reviews·Janet M PaveseRaymond C Bergan
Jan 24, 2014·International Journal of Molecular Sciences·Jane C QuinnLeslie A Weston
Feb 9, 2013·Critical Reviews in Food Science and Nutrition·Fengfeng WuXueming Xu
May 9, 2015·Seminars in Cancer Biology·Abbas K SamadiXujuan Yang
Mar 20, 2012·Journal of Photochemistry and Photobiology. B, Biology·Su-juan ZhangXiao Liu
Jan 29, 2014·Cell Biology International·Rafael de Paula RodriguesNewton Soares da Silva
Nov 13, 2015·Critical Reviews in Food Science and Nutrition·Qixing NieMingyong Xie
Jun 27, 2015·Journal of Photochemistry and Photobiology. B, Biology·A PopovicL M Davids
Jan 24, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Martina PilatovaJan Mojzis
Jan 18, 2017·Nutrition and Cancer·Wissam Zam, Aziz Khadour
Jul 1, 2017·Photodiagnosis and Photodynamic Therapy·Blassan P GeorgeNanjundaswamy M Hemmaragala
Nov 10, 2011·Pharmaceuticals·Peter SolárPeter Fedoročko
Dec 11, 2014·World Journal of Clinical Oncology·María Julia LambertiViviana Alicia Rivarola
Oct 19, 2013·EcoHealth·Ausraful IslamStephen P Luby
Jun 17, 2010·The Journal of Pharmacology and Experimental Therapeutics·Xiaoli WangYao Li
Jul 10, 2019·International Journal of Molecular Sciences·Manuel Gallardo-VillagránBruno Therrien
Dec 24, 2010·Radiation Research·Peter SolárPeter Fedoročko
Dec 31, 2019·Seminars in Cancer Biology·Ji Hoon JungSung-Hoon Kim
Jul 4, 2020·Anti-cancer Agents in Medicinal Chemistry·Giftson J SenapathyHeidi Abrahamse
Apr 2, 2021·The Journal of Pharmacy and Pharmacology·Xiaoxv DongLuis J Cruz
Mar 8, 2012·Chemical Research in Toxicology·Ana R M SoaresAngeles Juarranz

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