Down-regulation of beta2-adrenergic receptor expression by exercise training increases IL-12 production by macrophages following LPS stimulation

Biochemical and Biophysical Research Communications
Choh-Ei ItohHideki Ohno

Abstract

Three-week exercise training decreased the steady state level of beta(2)-adrenergic receptor (beta(2)AR) mRNA in peritoneal macrophages from BALB/c mice. When peritoneal macrophages from both exercise-trained and sedentary control mice were stimulated with lipopolysaccharide (LPS), interleukin (IL)-12 mRNA and protein expression was markedly higher in trained mice than in control mice. To determine whether enhanced production of IL-12 was associated with decreased expression of beta(2)AR, we transfected the macrophage cell line, RAW264, with a eukaryotic expression vector containing beta(2)ar cDNA, establishing a cell line overexpressing beta(2)AR (RAWar). Following LPS stimulation, IL-12 mRNA and protein expression was significantly lower in RAWar cells than in RAW264 cells transfected with vector alone (RAWvec). Furthermore, when the expression of transfected beta(2)AR in RAWar cells was down-regulated by a tetracycline repressor-regulated mammalian expression system, expression of IL-12 mRNA and protein following LPS stimulation tended to return to the levels in RAWvec cells. These findings indicate that macrophage production of IL-12 following LPS stimulation is regulated by the expression level of beta(2)AR, suggesting tha...Continue Reading

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Citations

Jul 31, 2009·Pflügers Archiv : European journal of physiology·Ken ShiratoHideki Ohno
Jan 18, 2016·The International Journal of Neuropsychopharmacology·Martina M HughesAndrew Harkin
Jun 19, 2012·Journal of Bodywork and Movement Therapies·Hamid MarefatiMahabat Mohseni
Oct 30, 2018·Journal of the American Heart Association·Richard P SloanKevin J Tracey

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