Down-regulation of beta3 adrenoreceptor gene expression in brown fat cells is transient and recovery is dependent upon a short-lived protein factor.

The Journal of Biological Chemistry
T BengtssonB Cannon

Abstract

The regulation of the expression of the beta3 adrenoreceptor gene was examined in the brown adipose tissue of intact mice and in murine brown fat primary cell cultures. Both in vivo and in vitro, high levels of beta3 receptor mRNA were observed. Acute cold exposure of mice resulted in a marked and rapid down-regulation of beta3 gene expression; this down-regulation was, however, transient. Similarly, in brown fat cell cultures, norepinephrine addition led to down-regulation of beta3 gene expression, with a lag phase of 30 min and with an apparent half-life of beta3 mRNA of approximately 30 min. This down-regulation was stimulated via the beta3 receptors themselves and mediated via cAMP; the apparent affinity of norepinephrine was extremely high (<1 nM). The degradation rate after actinomycin was identical to that after norepinephrine and was not affected by the presence of norepinephrine; thus, the down-regulation was due to cessation of transcription but not to an increased rate of degradation. Notably, inhibition of protein synthesis by cycloheximide also led to down-regulation. The norepinephrine-induced down-regulation was transient; spontaneous recovery occurred after approximately 18 h and was not due to depletion of adre...Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R F ThomasS B Liggett
Jul 16, 1990·Biochemical and Biophysical Research Communications·C Hough, D M Chuang
Jan 1, 1991·Annual Review of Physiology·S CollinsR J Lefkowitz
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N J IzzoW S Colucci
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S CollinsR J Lefkowitz
Sep 8, 1989·Science·L J EmorineA D Strosberg
Oct 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J L Hargrove, F H Schmidt
Dec 1, 1987·The American Journal of Physiology·M NéchadB Cannon
Jan 1, 1988·Annual Review of Cell Biology·J L BenovicR J Lefkowitz
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J R Hadcock, C C Malbon
Jan 1, 1986·Annals of Nutrition & Metabolism·J F AndrewsP Trayhurn
Mar 1, 1982·The American Journal of Physiology·J Nedergaard
May 1, 1982·The Journal of Clinical Investigation·J B YoungL Landsberg
Apr 1, 1995·Molecular and Cellular Endocrinology·S KlausD Ricquier
Apr 1, 1995·The American Journal of Physiology·J G Granneman, K N Lahners
May 5, 1995·The Journal of Biological Chemistry·B FèveA D Strosberg
Jan 1, 1995·Cellular Signalling·J G Granneman
Feb 1, 1995·Biochemical Society Transactions·S MarulloM Bouvier
Jan 1, 1995·British Journal of Pharmacology·F D'AllaireL J Bukowiecki
Oct 1, 1994·The American Journal of Physiology·J ZhaoJ Nedergaard
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·B FèveJ Pairault
Apr 1, 1993·Journal of Molecular Endocrinology·J P RevelliJ P Giacobino
Jan 1, 1993·Journal of Neurochemistry·J R Hadcock, C C Malbon

❮ Previous
Next ❯

Citations

Jun 13, 2013·Naunyn-Schmiedeberg's Archives of Pharmacology·Martina B Michel-Reher, Martin C Michel
Mar 22, 2000·British Journal of Pharmacology·D S HutchinsonR J Summers
Nov 12, 2013·Naunyn-Schmiedeberg's Archives of Pharmacology·Martin C Michel
Mar 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Luigi BellocchioGiovanni Marsicano
Apr 24, 2012·Trends in Endocrinology and Metabolism : TEM·Parth Patwari, Richard T Lee
Apr 29, 2016·Scientific Reports·Adel PezeshkiPrasanth K Chelikani
May 27, 2008·British Journal of Pharmacology·E DavisR J Summers
Feb 28, 2019·British Journal of Pharmacology·Katerina OkekeMartin C Michel
Jun 10, 2010·American Journal of Physiology. Endocrinology and Metabolism·Charlotte L MattssonBarbara Cannon
Mar 15, 2019·EMBO Reports·Montserrat CairóFrancesc Villarroya
Jun 4, 2014·Molecular Pharmacology·Hana CerneckaMartin C Michel
Nov 26, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Marie E GibbsSuzanne L Miller
Apr 20, 2000·The Journal of Biological Chemistry·J M LindquistJ Nedergaard
Aug 7, 1999·The Journal of Biological Chemistry·H M Guardiola-DiazS E Alexson
Dec 23, 1999·The Journal of Biological Chemistry·G E BronnikovJ Nedergaard
Aug 21, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jung A WooStephen B Liggett
Jan 13, 2004·Physiological Reviews·Barbara Cannon, Jan Nedergaard
Oct 17, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Irina ShabalinaJan Nedergaard
Nov 21, 2020·Scientific Reports·Francesca-Maria RaffaelliJens Mittag
Aug 23, 2005·European Journal of Pharmacology·Tadanori SugimotoYuichi Yokoyama

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.