Down-regulation of c-Met and Bcl2 by microRNA-206, activates apoptosis, and inhibits tumor cell proliferation, migration and colony formation

Oncotarget
Chengcao SunDejia Li

Abstract

Hsa-miRNA-206 (miR-206), highly expressed in skeletal muscle, has recently been discovered to have anticancer properties in different tissues. However, the role of miR-206 on lung cancer is still ambiguous. In this study, we investigated the role of miR-206 on the development of lung cancer. The results indicated that miR-206 expression was suppressed in lung cancer tissues and very low levels were found in non-small cell lung cancer (NSCLS) cell lines. Transient transfection of miR-206 into cultured A549 and SK-MES-1 cells led to significant decrease in cell growth, migration, invasion and colony formation, and promoted cell apoptosis. Using bioinformatics, we identified putative miR-206 binding sites within the 3'-untranslated region (3'-UTR) of the human c-Met and Bcl2 mRNA. The expression of c-Met and Bcl2 proteins were shown to be down-regulated after treated with miR-206 by subsequent Western blot and qRT-PCR analysis. Conversely, up-regulation of c-Met and Bcl2 were confirmed in tissue samples of human lung cancer, with its level inversely correlated with miR-206 expression. In addition, miR-206 also decreased the gene expression of MMP-9, CCND1 and CCND2 while increased the gene expression of p57 (Kip2) in A549 and SK-M...Continue Reading

References

Dec 5, 2002·Essays in Biochemistry·Yoshifumi Itoh, Hideaki Nagase
Dec 10, 2002·Annual Review of Medicine·Yoshitaka SekidoJohn D Minna
Apr 3, 2004·Trends in Molecular Medicine·Mark E Ewen, Justin Lamb
Jun 10, 2005·Nature·Jun LuTodd R Golub
Oct 1, 2005·Cancer Investigation·Janessa J Laskin, Alan B Sandler
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Jun 1, 2006·Nature Genetics·Nikolaus Rajewsky
Jul 25, 2006·Nature Reviews. Cancer·Carla Boccaccio, Paolo M Comoglio
Jul 29, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Massimiliano Mazzone, Paolo M Comoglio
Aug 4, 2006·Cancer Research·George Adrian Calin, Carlo Maria Croce
Oct 10, 2006·Oncogene·G A Calin, C M Croce
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Feb 24, 2007·Current Opinion in Genetics & Development·Ryusuke Niwa, Frank J Slack
Apr 3, 2007·Nature Genetics·Madhu S KumarTyler Jacks
Jul 4, 2007·Journal of Cellular Physiology·Silvia Benvenuti, Paolo M Comoglio
Sep 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Muller FabbriCarlo M Croce
Jan 17, 2008·Nature Reviews. Genetics·Witold FilipowiczNahum Sonenberg
Mar 4, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Federico CappuzzoMarileila Varella-Garcia
Aug 28, 2009·The Journal of Biological Chemistry·Dongsheng YanLili Tu
Sep 3, 2009·The Journal of Biological Chemistry·Guisheng SongLi Wang
Oct 16, 2009·The New England Journal of Medicine·Richard S HotchkissPaul E Swanson
Jun 11, 2010·Annual Review of Biochemistry·Marc Robert FabianWitold Filipowicz
Nov 26, 2010·Journal of Experimental & Clinical Cancer Research : CR·Zhe ChenJian Hu
Dec 16, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Xiaochen WangJun Zhao
Jan 20, 2011·Nature Reviews. Genetics·Eric Huntzinger, Elisa Izaurralde
Feb 5, 2011·Science·Sergej DjuranovicRachel Green
Mar 8, 2013·Biochemical and Biophysical Research Communications·Jing ZhouGuozhong Ji
Oct 11, 2013·Cell Cycle·Alessandra AlteriMilena Grossi
Dec 3, 2013·Pigment Cell & Melanoma Research·Robert W GeorgantasKoustubh Ranade
Jul 23, 2014·Seminars in Hematology·Mary Ann AndersonAndrew Roberts
Sep 14, 2014·Molecular Cancer·Sweta MishraLu-Zhe Sun
Oct 15, 2014·Cancers·Francesco GelsominoMarcello Tiseo
Feb 14, 2015·International Journal of Biological Sciences·Guoda MaKeshen Li

❮ Previous
Next ❯

Citations

Nov 15, 2016·Journal of Experimental & Clinical Cancer Research : CR·Mahmoud AlhosinChristian Bronner
Jun 24, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Tian LiXianhan Jiang
Jan 25, 2018·International Journal of Molecular Sciences·Jia Cui, William J Placzek
Sep 12, 2019·Technology in Cancer Research & Treatment·Yu ZhouYunli Zhou
Nov 29, 2016·Nature Reviews. Nephrology·Mark R Morris, Farida Latif
Aug 26, 2018·International Journal of Cancer. Journal International Du Cancer·Umberto MiglioAnna Sapino
Mar 29, 2020·Frontiers in Cell and Developmental Biology·Hong ZhanJun Lin
Nov 7, 2017·Oxidative Medicine and Cellular Longevity·Eman A ToraihAly A M Shaalan
Oct 31, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Yaping Zhu, Shurui Bu
Jun 16, 2017·Cancer Cell International·Ai-Hua ChenManzhen Zuo
Jul 15, 2017·International Journal of Molecular Sciences·Renqiao TangJianxin Wu
Apr 23, 2020·International Journal of Molecular Sciences·Ruben Mercado SantosWen Cai Zhang
May 3, 2019·The Journal of Pharmacy and Pharmacology·Ziyan GuoJianqing Yu
Jul 21, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ravand SamaeekiaHuiping Liu
May 29, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chunxiu DaiZhengzhong Yuan
Aug 10, 2016·Biochemical and Biophysical Research Communications·Jing FangCheng Gong
Feb 20, 2020·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Naizhi WangQin Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
protein assay
Assay
fluorescence microscopy
ELISA
xenograft
transfecting

Software Mentioned

PicTar
TargetScan
Image
Quantity One
CellQuest
SPSS
Pro Plus

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.