PMID: 7537973Apr 26, 1995Paper

Down-regulation of CFTR expression in an HT29-derived cell line by stable antisense RNA production

Biochimica Et Biophysica Acta
C J Wardle, A Harris

Abstract

In order to examine the interactions of the cAMP activated CFTR chloride ion channel with other chloride ion channels present in epithelial cells we have generated a model based on antisense mRNA down regulation of CFTR expression. The HT29-derived epithelial cell line CJC4-1 has integrated a plasmid that is constitutively expressing CFTR exons 1-6 in the antisense orientation, from the MoMULV LTR. This cell line shows a reduction in cAMP-activated chloride efflux, as measured by iodide efflux assay, in comparison to the parental HT29 cell line and a sense transfected control.

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J A WagnerP Gardner
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E J SorscherR D LeBoeuf
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Apr 1, 1973·Virology·F L Graham, A J van der Eb
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·L L ClarkeR C Boucher

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Citations

Mar 1, 1997·Journal of Cellular Physiology·C Montrose-RafizadehM H Montrose
Apr 20, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Susan R RheingoldUNKNOWN Children's Oncology Group Study
Mar 17, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Charles M RudinEverett E Vokes

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