Down-Regulation of ClC-3 Expression Reduces Epidermal Stem Cell Migration by Inhibiting Volume-Activated Chloride Currents

The Journal of Membrane Biology
Rui GuoChuan Cao

Abstract

ClC-3, a member of the ClC chloride (Cl(-)) channel family, has recently been proposed as the primary Cl(-) channel involved in cell volume regulation. Changes in cell volume influence excitability, contraction, migration, pathogen-host interactions, cell proliferation, and cell death processes. In this study, expression and function of ClC-3 channels were investigated during epidermal stem cell (ESC) migration. We observed differential expression of CLC-3 regulates migration of ESCs. Further, whole-cell patch-clamp recordings and image analysis demonstrated ClC-3 expression affected volume-activated Cl(-) current (I Cl,Vol) within ESCs. Live cell imaging systems, designed to observe cellular responses to overexpression and suppression of ClC-3 in real time, indicated ClC-3 may regulate ESC migratory dynamics. We employed IMARIS software to analyze the velocity and distance of ESC migration in vitro to demonstrate the function of ClC-3 channel in ESCs. As our data suggest volume-activated Cl(-) channels play a vital role in migration of ESCs, which contribute to skin repair by migrating from neighboring unwounded epidermis infundibulum, hair follicle or sebaceous glands, ClC-3 may represent a new and valuable target for stem ce...Continue Reading

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Citations

May 31, 2018·Physiological Reviews·Thomas J Jentsch, Michael Pusch
Oct 30, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Jie ZhongBin Xu
May 13, 2021·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Jul 16, 2021·Frontiers in Molecular Neuroscience·Rithvik R NalamalapuMargaret S Saha

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