Down-regulation of early sea urchin histone H2A gene relies on cis regulative sequences located in the 5' and 3' regions and including the enhancer blocker sns

Journal of Molecular Biology
Domenica Di CaroGiovanni Spinelli

Abstract

The tandem repeated sea urchin alpha-histone genes are developmentally regulated by gene-specific promoter elements. Coordinate transcription of the five genes begins after meiotic maturation of the oocyte, continues through cleavage, and reaches its maximum at morula stage, after which these genes are shut off and maintained in a silenced state for the life cycle of the animal. Although cis regulative sequences affecting the timing and the level of expression of these genes have been characterized, much less is known about the mechanism of their repression. Here we report the results of a functional analysis that allowed the identification of the sequence elements needed for the silencing of the alpha-H2A gene at gastrula stage. We found that important negative regulative sequences are located in the 462 bp sns 5 fragment located in the 3' region. Remarkably, sns 5 contains the sns enhancer blocking element and the most 3' H2A codons. In addition, we made the striking observation that inhibition of the anti-enhancer activity of sns, by titration of the binding proteins in microinjected embryos, also affected the capability of sns 5 to down-regulate transgene expression at gastrula stage. A further sequence element essential fo...Continue Reading

References

Jan 1, 1979·Annual Review of Biochemistry·L H Kedes
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Grunstein
Oct 1, 1976·Nucleic Acids Research·S G ClarksonM L Birnstiel
May 1, 1992·Molecular and Cellular Biology·R Kellum, P Schedl
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·F PallaG Spinelli
Dec 20, 1982·Nucleic Acids Research·G SpinelliI Di Liegro
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R Grosschedl, M L Birnstiel
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·F PallaG Spinelli
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F PallaG Spinelli
Apr 1, 1997·Current Opinion in Genetics & Development·P K Geyer
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·F PallaG Spinelli
Dec 10, 1999·Cell·E MaldonadoD Reinberg
Jun 21, 2001·Biochemical and Biophysical Research Communications·P Di SimoneG Spinelli
Nov 9, 2001·Annual Review of Genetics·T I Gerasimova, V G Corces
Feb 5, 2002·Genes & Development·Adam G WestGary Felsenfeld
Aug 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bonnie Burgess-BeusseGary Felsenfeld
Sep 5, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Giovanni Spinelli, Max L Birnstiel
Jan 24, 2003·Nature·Gary Felsenfeld, Mark Groudine
Feb 6, 2003·Cellular and Molecular Life Sciences : CMLS·P K Geyer, I Clark
Jun 6, 2003·Current Opinion in Cell Biology·Emily J Kuhn, Pamela K Geyer

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Citations

Apr 9, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Danilo D'ApolitoSantina Acuto
Sep 28, 2005·Blood Cells, Molecules & Diseases·Santina AcutoGiovanni Spinelli
Jun 18, 2020·International Journal of Molecular Sciences·Chiara Reina, Vincenzo Cavalieri

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