Down-regulation of Filamin A interacting protein 1-like Is associated with promoter methylation and an invasive phenotype in breast, colon, lung and pancreatic cancers [corrected

PloS One
Mijung KwonSteven K Libutti

Abstract

Identifying key mediators of cancer cell invasion and metastasis is critical to the development of more effective cancer therapies. We previously identified Filamin A interacting protein 1-like (FILIP1L) as an important inhibitor of cell migration and invasion in ovarian cancer. FILIP1L expression was inversely correlated with the invasive potential of ovarian cancer cell lines and ovarian cancer specimens. We also demonstrated that DNA methylation in the FILIP1L promoter was a mechanism by which FILIP1L was down-regulated in ovarian cancer. In our present study, we tested this observation in other cancer histologies: breast, colon, lung and pancreatic cancers. Both mRNA and protein expression of FILIP1L were down-regulated in these cancer cells compared with their normal epithelial cells. As in ovarian cancer, DNA methylation is a mechanism by which FILIP1L is down-regulated in these cancer histologies. Methylation status of the FILIP1L promoter was inversely correlated with FILIP1L expression. Reduced methylation in the FILIP1L promoter following treatment with a DNA demethylating agent was associated with restoration of FILIP1L expression in these cancer cells. Further, FILIP1L expression was inversely correlated with the in...Continue Reading

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Citations

Dec 12, 2013·International Journal of Cancer. Journal International Du Cancer·Mijung KwonSteven K Libutti
Mar 22, 2015·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Mircea BeuranSorin Hostiuc
Feb 27, 2015·World Journal of Gastroenterology : WJG·Zi-Qiang TianGui-Ying Wang

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Methods Mentioned

BETA
PCR
transfection

Software Mentioned

Mass Array
EpiTYPER
Gen5
GraphPad Prism

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