Down-regulation of flavonoid 3'-hydroxylase gene expression by virus-induced gene silencing in soybean reveals the presence of a threshold mRNA level associated with pigmentation in pubescence

Journal of Plant Physiology
Atsushi NagamatsuAkira Kanazawa

Abstract

Changes in flavonoid content are often manifested as altered pigmentation in plant tissues. Two loci have been identified as controlling pigmentation in soybean pubescence. Of these, the T locus appears to encode flavonoid 3'-hydroxylase (F3'H) protein: the T and t alleles are associated with tawny and gray colors, respectively, in pubescence. We previously down-regulated F3'H gene expression by virus-induced gene silencing (VIGS) in soybean. Despite this successful VIGS, the tawny pubescence pigmentation proved to be unchanged in greenhouse-grown plants. We hypothesized that the reduced mRNA level of the F3'H gene resulting from VIGS remained high enough to induce pigmentation. To verify this hypothesis, in the present study, we performed F3'H VIGS on plants grown under controlled conditions, in which the steady-state mRNA level of the F3'H gene was reduced to approximately 5% of that of greenhouse-grown plants. This VIGS treatment resulted in the loss of tawny pigmentation in pubescence, suggesting that the sf3'h1 gene is involved in the control of pigmentation in pubescence. We detected a marked decrease in target mRNA, an accumulation of short interfering RNAs (siRNAs), and a decrease in quercetin content relative to kaempf...Continue Reading

References

Jun 11, 2002·Annual Review of Nutrition·Julie A Ross, Christine M Kasum
Jul 25, 2003·Phytochemistry·Oliver YuJoan T Odell
Aug 21, 2003·Journal of Agricultural and Food Chemistry·Annalisa RomaniDaniela Heimler
Mar 26, 2004·Nature Reviews. Molecular Cell Biology·Lynne E Maquat
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May 11, 2005·Trends in Plant Science·Ronald KoesFrancesca Quattrocchio
Sep 21, 2006·The Journal of Heredity·Tsukasa IwashinaRyoji Takahashi
Sep 4, 2007·Plant Biotechnology Journal·Atsushi NagamatsuAkira Kanazawa

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Citations

Mar 7, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yong Guo, Li-Juan Qiu
Nov 9, 2012·Breeding Science·Megumi Kasai, Akira Kanazawa
Jan 1, 2016·Plant Biotechnology·Keisuke TasakiMasumi Yamagishi
Dec 1, 2013·Functional Plant Biology : FPB·St Phanie PfliegerVal Rie Geffroy

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