PMID: 9190207Feb 1, 1997Paper

Down-regulation of G-protein-mediated Ca2+ sensitization in smooth muscle

Molecular Biology of the Cell
M C GongA V Somlyo

Abstract

Prolonged treatment with guanosine 5'-[gamma-thio]triphosphate (GTP gamma S; 5-16 h, 50 microM) of smooth muscle permeabilized with Staphylococcus aureus alpha-toxin down-regulated (abolished) the acute Ca2+ sensitization of force by GTP gamma S, AIF-4, phenylephrine, and endothelin, but not the response to phorbol dibutyrate or a phosphatase inhibitor, tautomycin. Down-regulation also abolished the GTP gamma S-induced increase in myosin light chain phosphorylation at constant [Ca2+] and was associated with extensive translocation of p21rhoA to the particulate fraction, prevented its immunoprecipitation, and inhibited its ADP ribosylation without affecting the immunodetectable content of G-proteins (p21rhoA, p21ras, G alpha q/11, G alpha i3, and G beta) or protein kinase C (types alpha, beta 1, beta 2, delta, epsilon, eta, theta, and zeta). We conclude that the loss of GTP gamma S- and agonist-induced Ca2+ sensitization through prolonged treatment with GTP gamma S is not due to a decrease in the total content of either trimeric (G alpha q/11, G alpha i3, and G beta) or monomeric (p21rhoA and p21ras) G-protein or protein kinase C but may be related to a structural change of p21rhoA and/or to down-regulation of its (yet to be ide...Continue Reading

References

Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T KitazawaA P Somlyo
Nov 1, 1994·Canadian Journal of Physiology and Pharmacology·M P WalshK G Morgan
Nov 17, 1994·Nature·A P Somlyo, A V Somlyo
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M C GongA P Somlyo
Mar 1, 1996·The Journal of Biological Chemistry·K IchikawaD J Hartshorne

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Citations

Jan 18, 2003·Current Hypertension Reports·Karl SwärdMichael P Walsh
Sep 26, 1997·Nature Structural Biology·Y WeiZ S Derewenda
Mar 15, 2000·Protein Science : a Publication of the Protein Society·P W ReadR K Nakamoto
Jul 16, 2011·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Hiroyasu SakaiMiwa Misawa
Aug 5, 2000·Biochemical and Biophysical Research Communications·T HamaguchiT Nakano
Jul 23, 2014·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Hiroyasu SakaiYoshihiko Chiba
Mar 18, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yuichiro KikkawaKatsuya Hirano
Jan 15, 1999·Acta Physiologica Scandinavica·A P Somlyo, A V Somlyo
Mar 7, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·G P van Nieuw Amerongen, V W van Hinsbergh
Jun 10, 2010·Expert Review of Cardiovascular Therapy·Shripada RaoSanjay Patole
Nov 7, 1998·The Journal of Biological Chemistry·K MissyB Payrastre
Apr 18, 1997·The Journal of Biological Chemistry·M C GongA P Somlyo
Jun 17, 2003·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ignace MpioJean-Pierre Fauvel
Nov 4, 2000·Journal of Biomedical Science·D G JohnsR C Webb
Aug 16, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·H YoshimuraD O Warner
Jan 1, 2013·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Masatoshi Yumoto, Masaru Watanabe
May 26, 1999·American Journal of Respiratory Cell and Molecular Biology·A YoshiiM Mori
Jun 13, 2001·American Journal of Physiology. Cell Physiology·M C GongA P Somlyo
Sep 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Rebecca W CarterNancy L Kanagy
Jul 10, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L A WalkerA P Somlyo
Jun 12, 1998·Biochimica Et Biophysica Acta·E A HodsonJ S Lymn

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