Down-regulation of GABA(A) receptor via promiscuity with the vasoactive peptide urotensin II receptor. Potential involvement in astrocyte plasticity.

PloS One
Laurence DesruesHélène Castel

Abstract

GABA(A) receptor (GABA(A)R) expression level is inversely correlated with the proliferation rate of astrocytes after stroke or during malignancy of astrocytoma, leading to the hypothesis that GABA(A)R expression/activation may work as a cell proliferation repressor. A number of vasoactive peptides exhibit the potential to modulate astrocyte proliferation, and the question whether these mechanisms may imply alteration in GABA(A)R-mediated functions and/or plasma membrane densities is open. The peptide urotensin II (UII) activates a G protein-coupled receptor named UT, and mediates potent vasoconstriction or vasodilation in mammalian vasculature. We have previously demonstrated that UII activates a PLC/PIPs/Ca(2+) transduction pathway, via both G(q) and G(i/o) proteins and stimulates astrocyte proliferation in culture. It was also shown that UT/G(q)/IP(3) coupling is regulated by the GABA(A)R in rat cultured astrocytes. Here we report that UT and GABA(A)R are co-expressed in cerebellar glial cells from rat brain slices, in human native astrocytes and in glioma cell line, and that UII inhibited the GABAergic activity in rat cultured astrocytes. In CHO cell line co-expressing human UT and combinations of GABA(A)R subunits, UII mark...Continue Reading

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Citations

Nov 1, 2016·Molecular Neurobiology·Matthieu T CastexAnthony Falluel-Morel
Sep 4, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cédric BruléLaurent Prézeau
May 4, 2021·Frontiers in Cell and Developmental Biology·Vadim Le JoncourHélène Castel

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Methods Mentioned

BETA
irradiating
fluorescence
ELISA
transfection
flow cytometry
PCR

Software Mentioned

Microcal Origin
pClamp
Origin
Prism
Cell QuestTM

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