Down-regulation of heat-shock protein 27-induced resistance to photodynamic therapy in oral cancer cells

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Jisun KimOkjoon Kim

Abstract

Photodynamic therapy (PDT) of cells is a new treatment modality involving selective delivery of a photosensitive dye into target cells, followed by visible light irradiation. PDT induces cell death by excessive ROS generation. The effects of multiple photosensitizers were owing to the difference in cell types involving sensitizer-specific protein changes linked to resistance. HSP27 is regulated in response to stress and is associated with apoptotic process. The effects of HSP27 on PDT resistance are controversial and unclear. The purpose of this study was to investigate the role of HSP27 down-regulation in the PDT-induced cells and HSP27 regulation in the resistance to PDT. KB cells transfected with HSP27 siRNA were exposed to hematoporphyrin (HP) followed by irradiation at 635 nm at an energy density of 4.5 mW/cm(2). After irradiation, the effects on HSP27 down-regulation were assessed by MTT assay, flow cytometry, confocal analysis, Western blotting and caspase activity. The results of this study showed that down-regulation of HSP27 restored cell survival in HP-PDT-induced apoptotic KB cells. HSP27 down-regulation attenuated PDT-induced apoptosis through caspase-mediated pathway in KB cells. Also, HSP27 silencing regulated Ba...Continue Reading

References

Jan 1, 1992·Photochemistry and Photobiology·B W Henderson, T J Dougherty
Oct 1, 1996·The Annals of Thoracic Surgery·J S McCaughanT E Williams
Jan 1, 1997·Dermatology : International Journal for Clinical and Investigative Dermatology·P WolfH Kerl
May 9, 2000·The Annals of Thoracic Surgery·A MaierF M Smolle-Jüttner
Nov 14, 2001·Journal of Photochemistry and Photobiology. B, Biology·J G HanlonG Singh
Feb 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stefanie B FlohéHubert Kolb
Apr 5, 2003·Immunobiology·Frederieke H van DuijnhovenPeter J K Kuppen
May 2, 2003·Nature Reviews. Cancer·Dennis E J G J DolmansRakesh K Jain
Jul 27, 2004·Proteomics·Cecilia SartoMarzia Galli-Kienle
Mar 9, 2005·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Hsin-Ming ChenChun-Pin Chiang
Jun 24, 2005·Transplantation·Arjang DjamaliBryan Becker
Jan 13, 2006·Cancer Chemotherapy and Pharmacology·E S M ChuJ Y Chen
Jun 22, 2006·Lasers in Surgery and Medicine·Sandra O GollnickPatricia Maier
Feb 21, 2007·Journal of the European Academy of Dermatology and Venereology : JEADV·P G Calzavara-PintonR Sala
Apr 6, 2007·The Journal of Dermatology·Junji HayamiTakeshi Horio
Aug 19, 2007·Biochimica Et Biophysica Acta·Esther BuytaertPatrizia Agostinis
May 7, 2009·Photomedicine and Laser Surgery·Hongran ChoiOkjoon Kim
Oct 31, 2009·Circulation Research·Se-Chan KimAnne A Knowlton
May 4, 2011·Photodermatology, Photoimmunology & Photomedicine·Sally Helen Ibbotson
Jul 12, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Hongyou ZhaoQun Chen
Nov 18, 2011·International Journal of Hematology·Huifang HuangYong Wu

❮ Previous
Next ❯

Citations

Feb 24, 2015·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Deepika Bablani PopliVatchala Rani
Dec 10, 2016·Medical Science Monitor Basic Research·Jason YoungKrystyna Konopka
Jul 30, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Matías E RodríguezViviana A Rivarola
Dec 12, 2017·Journal of Cellular Biochemistry·Flávia Cristina P RosinLuciana Corrêa
Jan 27, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Matías Exequiel RodríguezViviana Alicia Rivarola
Feb 9, 2021·Frontiers in Oncology·Waleska K MartinsTayana M Tsubone
Mar 20, 2021·Photodiagnosis and Photodynamic Therapy·Ana Carolina PagliaroneRicardo Carneiro Borra

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis